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![[Figure 2]](gb5122fig2mag.jpg)
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Figure 2
(a) An overview of the 2PP-3D printed mix-and-inject device (i.e. mixing-GDVN) consisting of a micromixer, a GDVN (type C) and a connective capillary in between. The capillary extension was chosen to be L2 = 28 mm. (b) A microscopy image showing lysozyme crystal delivery on the SPB/SFX instrument (10× magnification, NA 0.28, pixel size ∼0.65 µm). Upstream from the depicted X-ray interaction region, the crystals enter from the main channel of the mixing device at 7 µl min−1 and are flow-focused by pure water entering from the side channel at 70 µl min−1. Downstream, the 11-fold diluted sample then enters the GDVN (type C, 100 µm liquid orifice). With a helium pressure of 550 psi (Qg = 34 mg min−1), a liquid jet of 7.5 µm in diameter delivers the crystals into the X-ray focus. With our prediction formula [Fig. 4(b)], the jet velocity was determined to be 30.5 m s−1. (c) A microscopy image of the lysozyme crystal dispersion from the utilized sample reservoir showing the near-monodisperse microcrystals. (d) A background-corrected detector image of lysozyme diffraction from the same 11-fold dilution collected on the AGIPD 1M detector. The detector (pixel size is 200 µm × 200 µm) consists of four movable quadrants, each quadrant consisting of four static independent modules. Each module is 26 mm × 103 mm (128 × 512 pixels) large and consists of 2 × 8 ASICs (application-specific integrated circuits). The magnified region (green rectangle) shows Bragg reflections within 2 × 2 ASICs. |
![[Figure 2]](gb5122fig2.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
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