A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale

Yakovlev, M.A.; Vanselow, D.J.; Ngu, M.S.; Zaino, C.R.; Katz, S.R.; Ding, Y.; Parkinson, D.; Wang, S.Y.; Ang, K.C.; La Riviere, P.; Cheng, K.C.

doi:10.1107/S160057752101287X

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Figure 6
Adult D. magna stained with PTA showing sub-cellular resolution for different cell types and structures (a). Cell types and structures that can be visualized by customizing various stack thicknesses included: muscle striations (about 2 µm) on the labral muscles (b), ommatidia (Om), optic nerves (ON), optic lobe (OL) and brain (Br) (c), setae of the filter plates (d), nucleoli (yellow arrows, ∼2 µm diameter) in the gut epithelial cells (red arrows) (e), nucleoli (green arrows) in the fat cells (f) and yolk globules (g) in a developing embryo. Panels (a) to (c) were generated from maximum intensity projections (MIP) of 50 slices, panel (d) from an MIP of 100 slices to visualize thicker three-dimensional structures and panels (e) to (g) from an MIP of ten slices. An MIP of ten slices is equivalent to a 5 µm-thick histology slide.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 29| Part 2| March 2022| Pages 505-514

https://doi.org/10.1107/S160057752101287X

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