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Figure 4
Heart contour (white arrows) and ECG electrodes (a) and complete microbeam array (b) registered on radiosensitive Gafchromic film [the films are of the same size in (a) and (b)]. (c) Gamma H2AX immunostain showing the DNA double-strand breaks in the heart tissue. The bright green fluorescent dots represent nuclei harbouring the phospho­rylated histone H2A in response to DNA double-strand breaks. RV: right ventricle; LV: left ventricle. Scale bar = 500 µm. Asterisks in the enlargement indicate traces of DNA double-strand breaks in the microbeam paths. The γH2AX-positive nuclei between the microbeam paths could be partially due to differences between the patterns of muscle contraction at the time of irradiation and the time of sample fixation and partially contributed by the valley dose.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 29| Part 4| July 2022| Pages 1027-1032
https://doi.org/10.1107/S1600577522004489
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