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Figure 2
Experimental realization of the micropipette aspiration setup. (a) Rendering of the manipulators controlling the movement of the pipettes relative to the sample environment. (b) Close-up rendering of pipettes inserted into the sample solution. The inset shows the pipettes bringing two GUVs into contact. (c) PTFE-coated diagnostic slide with twelve 5.2 mm-diameter wells, fixing the positions of the sample droplets. (df) Two opposing pipettes (inclined tips with angle of 30°, 80 µm outer diameter, 15 µm inner diameter; GYNEMED, Germany) centered above the droplet and inserted into the droplet. (g) The assembly is moved into the focus of an optical microscope with images recorded by an optical network camera.

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