Figure 4
Holographic imaging at ID01. (a–d) Coffee grain aspirated by a pipette and used as a test object. (a) Optical microscope top view showing the sample reaching into the opening. (b) Hologram of the aspirated sample. (c) Hologram corrected by a flat field containing the pipette. (d) Nonlinear Fresnel Tikhonov phase reconstruction (Huhn et al., 2022; Lohse et al., 2020) (β/δ = 0.0286, lim1 = 10−4, lim2 = 3 × 10−1). (e) Hologram of GUV imaging attempt not yet showing sufficient contrast. Scale bars: 30 µm. |