Figure 4
PC 12 cells viability and morphology following exposure to SS THz radiation. (a) Optical micrographs (insets) of PC 12 cells exposed to SS THz radiation and the control. Scale bars: 5 µm. CLSM micrographs of viable PC 12 cells are shown in green – the cells were stained and imaged after exposure to SS THz; the untreated control is seen on the right. (b) Quantification of viable PC 12 cells. No statistically significant differences were observed among the two experimental groups (p = 0.28). (c) SEM micrographs depicting PC 12 cell morphology. Blebs were present in the outer membrane of the PC 12 cells in response to SS THz radiation, highlighted in the black insets by red arrows. (d) Quantification of the diameter d as shown in the insets (normal cell followed by blebbing). The average size of the blebs was 1.08 ± 0.49 µm. |