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Figure 4
HiTT imaging can be successfully performed on various sample types. (a)–(c) Projection image, reconstructed slice and 3D rendering of a paraffin-embedded 1 mm murine kidney punch biopsy. Anatomical structures like glomeruli (red), distal convoluted tubules (cyan) and proximal convoluted tubules (green) can be easily discerned. (d)–(f) Projection image, reconstructed slice and 3D rendering of a Arabidopsis thaliana stem. The zoom-in in (e) shows that individual cells can be easily segmented and labelled. (g)–(i) Projection image, reconstructed slice and 3D rendering of an osmium-stained and resin-embedded mid-gut of an Anopheles stephensi mosquito, which was infected with Plasmodium berghei. Oocysts formed by the parasites are clearly visible in (h) (yellow) and segmented in (i).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 31| Part 1| January 2024| Pages 186-194
https://doi.org/10.1107/S160057752300944X
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