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Figure 2
(a–c) Examples of reconstructed slices and (d–e) typical raw-data 0° and 180° radiogram images obtained from Zernike-nanoCT scans of zebrafish spines. Reconstructed slices in 3D data from P05 (a) and from ANATOMIX (b, c) beamlines contain prominent halo and shade-off artifacts (indicated by blue arrows). All reconstructions reveal the bone spine (large structure in the center), micro-voids and internal porosity (white arrows) that corresponds to the LCN typical of osteocytic bone. Black asterisks (*) indicate polymer-covered regions near the edges of samples (a) and (c). Halo and shade-off artifacts arise from a mixture of strong bright streaks or shadows due to interference effects at boundaries between different material densities mixed with variations in the contrast of such boundaries. The size and intensity of these artifacts emerge from a summation of different contrasts when the sample is radiographed from different perspectives. The 180° opposite (horizontally flipped) radiograms extracted from tomographic scans of two samples demonstrate how the contrast of identical features varies. White asterisks highlight regions that differ in contrast at 0° and 180° angles. |
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