Protocol using similarity score and improved shrink-wrap algorithm for better convergence of phase-retrieval calculation in X-ray diffraction imaging

Yoshida, S.; Harada, K.; Uezu, S.; Takayama, Y.; Nakasako, M.

doi:10.1107/S1600577523009864

Figure 7
Comparison of the performance of the ordinary and steered PR calculations for the diffraction patterns [(a) and (e)] from the nuclei of budding yeast. (b) The distribution of 160 maps at the 6600th HIO cycle in the ordinary (left panel) and steered (right panel) PR calculations as illustrated in Fig. 5

. Representative electron density maps are depicted with scale bars of 300 nm. (c) The frequency distributions of the similarity scores among the maps from a set of 160 ordinary (red line) and steered (blue line) PR calculations at the 6600th HIO cycle. (d) The distribution of the maps at the 6000th OSS cycle illustrated in the same manner as panel (b). The left panel shows the distributions of the OSS maps, which started from the support of one of the maps displaying the smallest similarity score among the three sets of 160 ordinary PR calculations. The right panel shows the distribution of OSS maps, which started from the support of one of the maps displaying the smallest similarity scored in the three sets of 160 steered calculations. Representative electron density maps are shown with scale bars of 300 nm. Panels (f)–(h) show the results for the diffraction pattern in (e) as panels (a)–(d).

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 31| Part 1| January 2024| Pages 113-128

https://doi.org/10.1107/S1600577523009864

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