Figure 4
Z-profile (a) and white-light images at the second (b), third (c) and fourth (d) harmonics of the optical amplitude signal of cotton cellulose deposited on a silicon substrate. The height and the optical signal profile (e) are taken along the red line shown in panels (a) and (b). The arrowhead in panel (a) points to the position from where the nano-FTIR spectrum (cf. Fig. 5) was collected. The scale bar in panels (a)–(d) represents 100 nm. The AFM probe oscillation frequency and the tapping amplitude in the experiment were 259 kHz and 25 nm, respectively. |