|
Figure 3
Comparison of automated and manual data collection at the PF. Manual (blue) and automated (red) data collections were compared under 13 different ligand-soaking conditions (nos. 1–13 on the horizontal axis) using crystals of the lipid kinase PI5P4Kβ. The crystals used in each condition were prepared under identical crystallization, cryoprotection and ligand-soaking conditions. Comparisons were based on the resolution at I/σ(I) values greater than 2.0. A single diffraction dataset was collected from each crystal, and no crystal was used more than once. No significant differences were observed between the automated and manual data collection, suggesting sufficient performance of the automated data collection. |