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Figure 4
In situ data collection and structure solution. (a) The diffractometer at BL-17A for in situ data collection. (b) Helical data collection from multiple metalloprotein crystals to obtain complete datasets while reducing radiation damage. The plate was oscillated from −15° to +15° (30° in total), with an oscillation angle of 0.25° per frame and an exposure time of 0.05 s per frame. In total, 14 crystals from six drops were used. (c) Data-processing statistics of the helical data collection. The data were merged using KAMO (Yamashita et al., 2018BB54) with a maximum resolution of 2.1 Å (I/σI > 2). (d) The 2Fo − Fc map of the protein solved using in situ data collection. The contour level is 3σ. The Rwork and Rfree values are 0.18 and 0.23, respectively.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 32| Part 3| May 2025|
https://doi.org/10.1107/S1600577525001407
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