Current and future perspectives for structural biology at the Grenoble EPN campus: a comprehensive overview

McCarthy, A.A.; Basu, S.; Bernaudat, F.; Blakeley, M.P.; Bowler, M.W.; Carpentier, P.; Effantin, G.; Engilberge, S.; Flot, D.; Gabel, F.; Gajdos, L.; Kamps, J.J.A.G.; Kandiah, E.; Linares, R.; Martel, A.; Melnikov, I.; Mossou, E.; Mueller-Dieckmann, C.; Nanao, M.; Nurizzo, D.; Pernot, P.; Popov, A.; Royant, A.; de Sanctis, D.; Schoehn, G.; Talon, R.; Tully, M.D.; Soler-Lopez, M.

doi:10.1107/S1600577525002012

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Figure 2
Workflow of the SOS pipeline for ESRF users at CM01: the pipeline, designed for users without regular access to a vitrification platform or screening microscope, follows the above structured approach at CM01, the cryo-EM beamline at ESRF. The workflow consists of five steps for liquid samples, beginning with negative-stain EM as a quality control step, and three steps for vitrified grids, starting directly with grid screening. Successful negative-stain EM leads to vitrification trials, followed by iterative cryo-EM grid screening to determine suitability for Krios data collection. Step 4 is currently performed by the user.

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ISSN: 1600-5775

Volume 32| Part 3| May 2025|

https://doi.org/10.1107/S1600577525002012

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