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Figure 2
Hologram and phase reconstructions of a catalytic particle. (a) Example showing one of the two normalized holographic intensity interference patterns I/I0 (hologram) of a catalytic particle at one tomographic angle. (b–d) Comparison of different phase retrieval methods and constraints. The reconstruction in (b) uses an unconstrained linear contrast transfer function (CTF). The reconstructions (c) and (d) are obtained using the HoToPy–Tikhonov algorithm. For these, a pixel-wise non-positivity constraint is used and for (d) additionally a finite disk-shaped support, indicated by the dashed circle. Scale bars: 5 µm. Effective pixel size: 17.2 nm. Images have 2160 × 2560 pixels. |
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