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Figure 2
FITC–dextran uptake by PC 12 cells exposed to variable intensity THz for 10 min. (a)–(c) CLSM micrographs of (a) untreated cells and (b) cells treated with HI THz and immediately placed in fluorescein dextran. No uptake is visible in control cells whereas exposed cells show internalized green fluorescence. The inset graph shows a fluorescence intensity profile for a single cell. (c) CLSM micrographs of PC 12 cells treated with HI THz and immediately placed in fluorescein dextran for 10 min and then washed. (d) FC scatter plots showing the population of FITC-positive single live cells for cells exposed to LI, MI and HI THz for 10 min, respectively. (e) Mean fluorescence intensity of cells exposed to increasing intensity of THz radiation and incubated (10 min) in fluorescein dextran, normalized to the control. No difference in fluorescence intensity of the cells was observed for LI–MI THz-exposed populations. (f) FITC SiO2 NS uptake per cell for PC 12 exposed to HI, MI and LI THz radiation, normalized to the control. Statistical significance is denoted by *** p < 0.01, ** p < 0.05, ns = non-significant. (g) CLSM analyses of FITC–SiO2 NPs in non-exposed PC 12 cells (top panel) and PC 12 cells following THz exposure (bottom panel). Yellow arrows indicate NPs. |
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