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Figure 1
Illustrative example of our X-ray-histology application, with (real) paired toluidine-blue stained histology (a) and SR-µCT (b) images of an Mg–10Gd screw implant in bone extracted after eight weeks in vivo. In the histology, the green line marks the interface between the residual screw and degradation layer. In the µCT slice, the red line indicates the boundary between the degradation layer and surrounding bone. The residual screw appears black in histology due to total light attenuation by the metal. Regions identified as the degradation layer in histology correspond to areas in µCT with mildly differing X-ray attenuation to the residual screw. Higher magnification views (c, d) indicated by the orange boxes highlight the areas surrounding the degradation layer. Arrows in black (c) or white (d) point to regions of new (woven) bone, appearing blue in histology and exhibiting lower attenuation values in µCT due to lower mineralization content. Figure adapted from Krüger et al. (2022) |

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