Design and commissioning of a new synchrotron beamline dedicated to X-ray footprinting mass spectrometry

Gupta, S.; Russell, B.; Kristensen, L.G.; de Chant, J.; Lu, A.; Obst-Huebl, L.; Rad, B.; Tyler, J.; Subramanian, S.; Kidd, S.; Paul, S.; Chen, Y.; Petzold, C.J.; Kahan, D.N.; Costello, S.M.; Nakamura, K.; Inman, J.L.; MacDowell, A.A.; Spucces, A.; Ralston, C.Y.

doi:10.1107/S1600577526003711

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Figure 5
Experimental dose calculation. (a) The top panel shows images of the beam and the shadows of the 100 µm jet and 200 µm ID capillary on the Nd-YAG screen as captured by the BAM. The bottom panel shows the segmented beam using a 460 µm vertical X-ray slit at the four selected positions, P1 to P4. (b) Vertical and horizontal beam intensity profile determined using ImageJ for the full and truncated beam, respectively. (c) Hydroxyl radical reactivity rate for Alexa488 exposed at positions P1 through P4 using the 100 µm jet and the 200 µm ID capillary. (d) Example of dose response data of residue M44 using the 75 µm jet and 200 µm ID capillary for free SpyCatcher003 (black) and SpyCatcher003–SpyTag001 complex (red). Both jet and capillary flow showed a similar ratio of modification (R) at residue M44 upon complex formation, where R represents the ratio of the hydroxyl radical reactivity rate between the free and complex states.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 33| Part 4| July 2026|

https://doi.org/10.1107/S1600577526003711

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