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Figure 9
Water structure in the central core of the T6 insulin hexamer in the 100 K dehydrated structure compared with that in the 120 K hydrated structure (PDB code 1mso ). In the 120 K hydrated structure, all atoms and bonds are reduced in size and the color scheme is as follows: in water layers 1, 2 and 3 water molecules are red, blue and green, respectively; the GluB13 side chains are red and the black dotted lines represent hydrogen-bonded interactions. In the 100 K dehydrated structure, the B-chain segments are green, layer 1 and 3 water molecules are yellow and cyan, respectively, and bonds in the GluB13 side chains are cyan. The cyan dotted line represents the three symmetry-related hydrogen bonds between waters 624 and 622 in the 100 K dehydrated structure. Prepared with the programs MOLSCRIPT (Kraulis, 1991 ) and Raster3D (Merritt & Bacon, 1997 ). |


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