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Figure 4
Calculated dose absorbed by a crystal of the protein PPK with and without the inclusion of Compton scattering. The calculations were performed assuming a crystal size of 0.04 × 0.1 × 0.05 mm, a top-hat-shaped beam of 0.05 × 0.05 mm with a photon flux of 1012 photons s−1 and an exposure time of 0.2 s per image. The protein consists of 398 residues, crystallizes in space group P1 with four monomers in the unit cell and contains three cysteines and 12 methionines (excluding the N-­terminal methionine residue) with approximately 58% solvent content. The lower panel is an enlargement of the 10–200 kGy dose region of the upper panel, highlighting the small difference between the absorbed dose with and without the inclusion of Compton scattering at higher incident energies.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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