Review panel for Acta Crystallographica Section F

The 1.75 Å resolution structure of fission protein Fis1 from Saccharomyces cerevisiae reveals elusive interactions of the autoinhibitory domain

A 1.75 Å resolution crystal structure of the Fis1 cytoplasmic domain from Saccharomyces cerevisiae is reported which adopts a tetratricopeptide-repeat fold.

Novel crystallization conditions for tandem variant R67 DHFR yield a wild-type crystal structure

A modification of previously published protocols for the crystallization of homotetrameric R67 DHFR was used to crystallize a mixed wild-type and variant tandem dimer of R67 DHFR. Surprisingly, a fully wild-type crystal structure was obtained, apparently as a consequence of selective proteolytic degradation of the variant.

A large conformational change in the putative ATP pyrophosphatase PF0828 induced by ATP binding

Crystal structures of the putative ATP pyrophosphatase PF0828 reveal a new domain (EGT domain), and a large conformational change upon ATP binding.

X-ray structure of the SH3 domain of the phosphoinositide 3-kinase p85β subunit

The crystal structure of the SH3 domain of the p85β subunit of human PI3K was determined to 2.0 Å resolution by molecular replacement. The overall structure is very similar to that of the p85α subunit of PI3K. The binding of two proline-rich ligand peptides to p85β SH3 was also characterized.

High-resolution X-ray study of the effects of deuteration on crystal growth and the crystal structure of proteinase K

A high-resolution X-ray crystallographic study of the effects of solvent deuteration on the crystallization of proteinase K shows negligibly small degradations of the crystals owing to solvent deuteration and small structural differences between nondeuterated and deuterated crystals of proteinase K.

Structure of the catalytic domain of Plasmodium falciparum ARF GTPase-activating protein (ARFGAP)

The crystal structure of the catalytic domain of ARF GTPase-activating protein (ARFGAP) of Plasmodium falciparum has been determined at 2.4 Å resolution and compared with the structures of mammalian ARFGAPs.

Structure of cellobiose phosphorylase from Clostridium thermocellum in complex with phosphate

The structural characterization of cellobiose phosphorylase from C. thermocellum reveals the residues involved in phosphate coordination and provides insight into substrate binding and discrimination.

Crystallographic analysis of the C-terminal domain of the Escherichia coli lipoprotein BamC

The assembly of bacterial outer membrane proteins is catalyzed by the BAM complex, which is made up of five proteins (BamA–E). Structural and bioinformatic analysis of the C-terminal domain of E. coli BamC (BamC_C) suggests that a negatively charged conserved surface on BamC_C may be an important protein–protein interaction site.

Purification, crystallization and diffraction studies of the methyltransferases BT_2972 and BVU_3255 from antibiotic-resistant pathogens of the genus Bacteroides from the human intestine

The expression, purification, crystallization and diffraction of two methyltransferases BT_2972 and BVU_3255 from two Bacteroides species of antibiotic-resistant pathogens from the human intestine are reported.

Expression, crystallization and preliminary X-ray characterization of the human epithelial cell-adhesion molecule ectodomain

The extracellular part of the human epithelial cell-adhesion molecule (EpCAM; CD326) was cloned, mutated to prevent N-linked glycosylation, expressed in insect cells, purified and crystallized. Diffraction data were collected to 1.95 Å resolution.

Crystallization and preliminary X-ray diffraction of the first periplasmic domain of SecDF, a translocon-associated membrane protein, from Thermus thermophilus

SecDF is a multi-path membrane protein required for efficient protein translocation and integration via the Sec translocon. Crystals of the first periplasmic domain of SecDF, an essential element for SecDF function, diffracted X-rays to 2.3 Å resolution.

Crystallization and preliminary crystallographic analysis of an Ig-domain-encompassing fragment of the giant adhesion protein SiiE from Salmonella enterica

SiiE from S. enterica is a giant adhesion molecule with a total of 5559 amino acids that is specifically required for initial contact between the pathogen and polarized epithelial cells. Using limited proteolysis, a fragment of SiiE that encompasses Ig domains 50–52 was produced and crystallized and diffraction data were collected to 1.85 Å resolution.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction crystallographic study of human synaptotagmin 5 C2A domain

This paper reports the cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of the first C2 domain of synaptotagmin 5.

Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3,4-di­chloroisocoumarin

A lipolytic enzyme of the SGNH-hydrolase family from S. rimosus was inhibited with 3,4-dichloroisocoumarin, crystallized and analyzed using X-ray diffraction.

Cloning, expression, purification, crystallization and preliminary X-ray analysis of the 31 kDa Vibrio cholerae heat-shock protein VcHsp31

A heat-shock protein from V. cholerae (VcHsp31) has been cloned, expressed, purified and crystallized. Crystals of VcHsp31 belonged to a monoclinic space group and diffracted to 1.9 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of di­hydrodipicolinate synthase 2 from Arabidopsis thaliana

Dihydrodipicolinate synthase (DHDPS) catalyzes the first committed step of the lysine-biosynthetic pathway in plants and bacteria. The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis (to 2.5 Å resolution) of DHDPS2 from A. thaliana are reported.

Crystallization and preliminary X-ray analysis of the RPB5 subunit of human RNA polymerase II

The recombinant RPB5 subunit of human RNA polymerase II has been crystallized by vapour diffusion in hanging drops.

Crystallization, high-resolution data collection and preliminary crystallographic analysis of Aura virus capsid protease and its complex with dioxane

A 17 kDa capsid protease domain from Aura virus was purified, crystallized together with its complex with dioxane and characterized by the X-ray diffraction method.

Crystallization and preliminary X-ray diffraction analysis of the human XRCC4–XLF complex

A complex of the human DNA-repair proteins XRCC4 and XLF was crystallized under high-salt and extreme dehydration conditions to produce diffraction to 3.9 Å resolution. Initial phasing information was obtained from molecular replacement with single-wavelength anomalous diffraction using tantalum bromide clusters.

Crystallization and preliminary X-ray crystallographic study of the human MST2 SARAH domain

The overproduction, purification, crystallization and preliminary X-ray analysis of the MST2 SARAH domain are described.

The macromolecular complex of ICP and falcipain-2 from Plasmodium: preparation, crystallization and preliminary X-ray diffraction analysis

The macromolecular complex of ICP (inhibitor of cysteine proteases) from P. berghei and falcipain-2 from P. falciparum has been prepared and crystallized, and a diffraction data set has been collected to a resolution of 2.6 Å.

Crystallization and preliminary crystallographic analysis of dUTPase from the φ11 helper phage of Staphylococcus aureus

The cloning, purification, crystallization and preliminary X-ray diffraction analysis of a novel staphylococcal phage dUTPase is reported. This protein contains a specific polypeptide insertion that is potentially responsible for modulation of expression of superantigenicity island genes.

Crystallization and preliminary X-ray diffraction analysis of an archaeal tRNA-modification enzyme, TiaS, complexed with tRNA^Ile2 and ATP

A. fulgidus TiaS was cocrystallized with tRNA^Ile2 and ATP and X-ray diffraction data were collected to 2.9 Å resolution using a synchrotron-radiation source.

Crystallization and preliminary X-ray analysis of the RXLR-type effector RXLR3 from the oomycete pathogen Hyaloperonospora arabidopsidis

RXLR3 is an oomycete effector from H. arabidopsidis. Crystals of the RXLR3 effector domain were obtained and X-ray data were recorded to a resolution of 1.8 Å.

Crystallization and preliminary X-ray crystallographic studies of the PYD domain of human NALP3

The PYD domain of human NALP3 was crystallized. The crystals were found to belong to the primitive monoclinic space group P2₁, with unit-cell parameters a = 42.03, b = 60.14, c = 51.61 Å, β = 107.40°. The crystals were obtained at 293 K and diffracted to a resolution of 1.7 Å.

Crystallization and preliminary X-ray analysis of a dye-linked D-lactate de­hydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix

A dye-linked D-lactate dehydrogenase from a hyperthermophilic archaeon was successfully isolated and crystallized.

Preliminary neutron crystallographic study of human transthyretin

Human transthyretin, a hormone-binding protein of high abundance in blood and cerebrospinal fluid, is intrinsically amyloidogenic. Preliminary results from a neutron diffraction analysis of this protein that may shed light on the mechanism of tetramer dissociation and successive fatal aggregation are presented.

Crystallization and preliminary X-ray crystallographic analysis of putative tRNA-modification enzymes from Pyrococcus furiosus and Thermus thermophilus

Two orthologous putative tRNA methyltransferases from P. furiosus and T. thermophilus have been expressed, purified and crystallized. X-ray diffraction data were collected to 2.2 and 2.05 Å, respectively.

Preliminary X-ray analysis of twinned crystals of the Q88Y25_Lacpl esterase from Lactobacillus plantarum WCFS1

The Q88Y25_Lacpl esterase from L. plantarum WCFS1 has been recombinantly expressed, purified and crystallized. A native diffraction data set has been collected to 2.24 Å resolution.

Purification, crystallization and preliminary X-ray analysis of the DndE protein from Salmonella enterica serovar Cerro 87, which is involved in DNA phosphoro­thioation

DndE is one of five essential proteins that are required for the DNA phosphorothioation process. However, its exact biochemical role in sulfur modification of DNA remains unclear. In this study, the DndE protein homologue from Salmonella enterica serovar Cerro 87 was overexpressed, purified and crystallized.

Crystallization and preliminary X-ray data collection of the L27_PATJ–(L27N,L27C)_Pals1–L27_MALS tripartite complex

Four different L27_PATJ–(L27N,L27C)_Pals1–L27_MALS constructs have been cloned, expressed, purified and crystallized. Crystals of tripartite complex 1 of L27_PATJ–(L27N,L27C)_Pals1–L27_MALS diffracted to 2.05 Å resolution.

Crystallization and preliminary X-ray diffraction crystallographic study of tRNA m¹A58 methyltransferase from Saccharomyces cerevisiae

The expression, purification, crystallization and X-ray diffraction analysis of tRNA m¹A58 methyltransferase from S. cerevisiae are reported.

HisB from Mycobacterium tuberculosis: cloning, overexpression in Mycobacterium smegmatis, purification, crystallization and preliminary X-ray crystallographic analysis

HisB from M. tuberculosis was cloned, overexpressed in M. smegmatis, purified and crystallized in three crystal forms.

Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate de­hydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate

The crystallization of glucose-6-phosphate dehydrogenase from the human pathogen T. cruzi is reported.

Crystallization and preliminary X-ray crystallographic analysis of ligand-free and arginine-bound forms of Thermotoga maritima arginine-binding protein

In this study, the hyperthermostable arginine-binding protein isolated from T. maritima has been crystallized in ligand-free and arginine-bound forms. Crystals of the apo and holo forms diffracted to 2.2 and 2.7 Å resolution, respectively.