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Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X

March 2012 issue

Highlighted illustration

Cover illustration: The crystal structure of the cytoplasmic cyclophilin A (CyPA) from the bacterium Azotobacter vinelandii complexed with a synthetic tetrapeptide determined by molecular replacement at 2 Å resolution (Christoforides et al., p. 259).

editorial


Acta Cryst. (2012). F68, 252
doi: 10.1107/S174430911200680X

scientific comment


structural communications


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The crystal structure of the cytoplasmic cyclophilin A (CyPA) from the bacterium Azotobacter vinelandii complexed with a synthetic tetrapeptide has been determined by molecular replacement at 2 Å resolution.

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Structures of IPMDH from the obligate piezophile S. benthica DB21MT-2 (SbIPMDH) and the nonpiezophile S. oneidensis MR-1 (SoIPMDH) were determined at atmospheric pressure. Comparison of the structures revealed that SbIPMDH is in a more open conformation and has a larger internal cavity volume than SoIPMDH.

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Large quantities of recombinant human carboxylesterase 1 have been produced in an economical whole insect larvae system. The crystal structure of this enzyme is essentially identical to that produced by cell culture techniques.

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A thermostable penicillin G acylase from A. faecalis has been crystallized in two space groups: C2221 and P41212. X-ray diffraction data were collected to 3.3 and 3.5 Å resolution, respectively.

crystallization communications



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A purified blue-light-absorbing proteorhodopsin D97N mutant protein (BPR_D97N) has been crystallized using the vapour-diffusion method.

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In order to characterize the type IV pili of nontypeable Haemophilus influenzae, an attempt to solve the atomic structure of the major pilin subunit PilA was initiated. A 1.73 Å resolution X-ray diffraction data set was collected from native N-terminally truncated PilA (ΔN-PilA).

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Succinic semialdehyde dehydrogenase (SSADH) from S. pyogenes was purified and crystallized. Crystals of native and NAD+-complexed SSADH diffracted to resolutions of 1.6 and 1.7 Å, respectively.

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Enoyl-acyl carrier protein reductase (FabK) from S. mutans strain UA159 was cloned, overexpressed, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.40 Å.

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Translation elongation factor eEF1A2 was purified to homogeneity from rabbit muscles. Obtained crystals of eEF1A2 diffracted to 2.5 Å resolution and belonged to space group P6122 or P6322.

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Casein kinase I-like protein from rice was crystallized. The crystals were found to belong to the monoclinic space group C2, with unit-cell parameters a = 108.83, b = 69.60, c = 55.85 Å, β = 109.47°. The crystals were obtained at 293 K and diffracted to a resolution of 2.0 Å.

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Crystals of the XccFimXEAL–c-di-GMP and SeMet-XccFimXEAL–c-di-GMP–XccPilZ complexes from X. campestris diffracted to resolutions of 2.5 and 2.7 Å, respectively.

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T. harzianum endoglucanase III was expressed in P. pastoris, purified and crystalized. A native X-ray diffraction data set was collected.

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Salmonella FlgA, a periplasmic chaperone essential for flagellar P-ring assembly, has been expressed and purified and the crystals have been characterized by X-ray diffraction.

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TPP3 is a class II plant defensin from tomato. Here, the expression, purification, crystallization and preliminary X-ray crystallographic analysis of recombinant TPP3 are reported in order to define its structure and function in relation to other class II plant defensins.

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Alginate importer from Sphingomonas sp. A1 is a member of the ABC transporter superfamily that directly transports alginate polysaccharide into the cytoplasm. Crystals of alginate importer in complex with the periplasmic binding protein AlgQ2 diffracted X-rays to 3.3 Å resolution.


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Cocrystallization of ferredoxin and glutamate synthase was successfully carried out and the resulting crystals were demonstrated to be suitable for structural determination of an electron-transfer complex of these two proteins.

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A hyperthermostable endoglucanase from P. furiosus expressed as a truncated form without the N-terminal amino acids was crystallized. The crystal diffracted to a resolution of 1.07 Å.


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The catalytic domain of human Dus2-like enzyme was purified and crystallized, and data were collected to 1.9 Å resolution.

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The crystallization conditions and preliminary crystal characterization of the cytoplasmic cyclic nucleotide-binding homology domain from the mouse EAG potassium channel are reported.

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The cytoplasmic domain of BRI1-associated kinase 1 from A. thaliana has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.6 Å resolution.

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The subclass B3 metallo-β-lactamase SMB-1 was crystallized by the hanging-drop vapour-diffusion method. Two types of crystals belonging to space group P31 were obtained.

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The L1 ribosomal protein from B. pseudomallei has been overexpressed, purified and crystallized in a form suitable for X-ray analysis.


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Residues 38–440 of CdpNPT from A. fumigatus were overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 2.5 Å.

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The SH2 domain of the human protein tyrosine kinase Fyn has been expressed, purified and crystallized in the unbound state and in complex with a high-affinity phosphotyrosine peptide.
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