data for structural and crystallization communications - example

Examples 2 and 3: different reservoir components

Crystallization 2 and 3: Lectin I (L-1) was concentrated to 10 mg/ml in 0.2 M glycine/HCl buffer pH 2.5 for setups. To obtain L-I crystals in complex with lactose and galactose, the sugar compound was added to the above mentioned buffer to a concentration of 0.2 M. Crystals of L-I complexed with lactose and galactose were obtained by hanging drop vapour diffusion. Droplets of 1 μl protein and 1 μl reservoir solutions were equilibrated against a reservoir of 0.75 ml of the reservoir solution (30-40% saturated ammonium sulfate in 0.1 M glycine/HCl buffer and 0.1 M lactose pH 2.5) to which was added in added 40 μl dioxane. The crystallization conditions for the L-I galactose complex differed only in the addition of 0.2 M galactose rather than lactose to the protein solution.
Cryo-conditions for crystals 2 and 3: The cryoprotectant solution contained 30-35% (v/v) glycerol, 40% saturated ammonium sulphate, 0.1 M lactose/galactose in 0.1 M glycine/HCl buffer at pH 2.5.

Example 2: lectin in complex with lactose

_exptl_crystal.crystal_id         2
_exptl_crystal.size_max           ?
_exptl_crystal.size_mid           ?
_exptl_crystal.size_min           ?

_exptl_crystal_grow.crystal_id    2
_exptl_crystal_grow.method        'hanging-drop vapour diffusion'
_exptl_crystal_grow.temp_details  'ambient'


loop_
_pdbx_exptl_crystal_grow_sol.crystal_id  
_pdbx_exptl_crystal_grow_sol.sol_id      
_pdbx_exptl_crystal_grow_sol.volume
_pdbx_exptl_crystal_grow_sol.volume_units
_pdbx_exptl_crystal_grow_sol.pH

2    'protein'       1.     'microlitre'   2.5
2    'precipitant'   1.     'microlitre'   2.5
2    'reservoir'     .79    'millilitre'   2.5

loop_
_pdbx_exptl_crystal_grow_comp.crystal_id
_pdbx_exptl_crystal_grow_comp.sol_id
_pdbx_exptl_crystal_grow_comp.comp_id
_pdbx_exptl_crystal_grow_comp.comp_name
_pdbx_exptl_crystal_grow_comp.conc
_pdbx_exptl_crystal_grow_comp.conc_range
_pdbx_exptl_crystal_grow_comp.conc_units

2  'protein'     1  'protein'           10   .       'milligrams_per_millilitre'
2  'protein'     2  'glycine/HCl'       0.1  .       'molar'
2  'protein'     3  'lactose/galactose' 0.2  .       'molar'

2  'reservoir'   1  'ammonium sulfate'  .    '30-40' 'percent_weight_by_volume'
2  'reservoir'   2  'glycine/HCl'       0.1  .       'molar'
2  'reservoir'   3  'lactose'           0.1  .       'molar'
2  'reservoir'   4  'dioxane'           5.0  .       'percent_volume_by_volume'

2  'precipitant' 1  'ammonium sulfate'  .    '30-40' 'percent_weight_by_volume'
2  'precipitant' 2  'glycine/HCl'       0.1  .       'molar'
2  'precipitant' 3  'lactose'           0.1  .       'molar'
2  'precipitant' 4  'dioxane'           5.0  .       'percent_volume_by_volume'

_pdbx_exptl_crystal_cryo_treatment.crystal_id   2

_pdbx_exptl_crystal_cryo_treatment.final_solution_details
; The cryoprotectant solution contained 30-35% (v/v) glycerol,
  40% saturated ammonium sulfate, 0.1M lactose/galactose in 0.1M
  glycine/HCl buffer at pH 2.5.
;

How this example will appear in the journal

Crystallization
Crystallization method Hanging-drop vapour diffusion  
Temperature Ambient
Crystallization solutions
   Protein (1 μl) Protein 10 mg ml-1, glycine/HCl 0.1 M, lactose/galactose 0.2 M, pH 2.5
   Precipitant (1 μl) ammonium sulfate 30-40% (w/v), glycine/HCl 0.1 M, lactose 0.1 M, dioxane 5.0% (w/v), pH 2.5
   Reservoir (0.79 ml) ammonium sulfate 30-40% (w/v), glycine/HCl 0.1 M, lactose 0.1 M, dioxane 5.0% (w/v), pH 2.5
Cryo treatment
  Final cryoprotection solution The cryoprotectant solution contained 30-35% (v/v) glycerol, 40% saturated ammonium sulfate, 0.1M lactose/galactose in 0.1M glycine/HCl buffer at pH 2.5


Example 3: lectin in complex with galactose

_exptl_crystal.crystal_id         3
_exptl_crystal.size_max           ?
_exptl_crystal.size_mid           ?
_exptl_crystal.size_min           ?

_exptl_crystal_grow.crystal_id    3
_exptl_crystal_grow.method        'hanging-drop vapour diffusion'
_exptl_crystal_grow.temp_details  'ambient'

loop_
_pdbx_exptl_crystal_grow_sol.crystal_id  
_pdbx_exptl_crystal_grow_sol.sol_id      
_pdbx_exptl_crystal_grow_sol.volume
_pdbx_exptl_crystal_grow_sol.volume_units
_pdbx_exptl_crystal_grow_sol.pH

3    'protein'       1.     'microlitre'   2.5
3    'precipitant'   1.     'microlitre'   2.5
3    'reservoir'     .79    'millilitre '  2.5

loop_
_pdbx_exptl_crystal_grow_comp.crystal_id
_pdbx_exptl_crystal_grow_comp.sol_id
_pdbx_exptl_crystal_grow_comp.comp_id
_pdbx_exptl_crystal_grow_comp.comp_name
_pdbx_exptl_crystal_grow_comp.conc
_pdbx_exptl_crystal_grow_comp.conc_range
_pdbx_exptl_crystal_grow_comp.conc_units

3  'protein'     1  'protein'           10   .       'milligrams_per_millilitre'
3  'protein'     2  'glycine/HCl'       0.1  .       'molar'
3  'protein'     3  'lactose/galactose' 0.2  .       'molar'

3  'reservoir'   1  'ammonium sulfate'  .    '30-40' 'percent_weight_by_volume'
3  'reservoir'   2  'glycine/HCl'       0.1  .       'molar'
3  'reservoir'   3  'galactose'         0.1  .       'molar'
3  'reservoir'   4  'dioxane'           5.0  .       'percent_volume_by_volume'

3  'precipitant' 1  'ammonium sulfate'  .    '30-40' 'percent_weight_by_volume'
3  'precipitant' 2  'glycine/HCl'       0.1  .       'molar'
3  'precipitant' 3  'galactose'         0.1  .       'molar'
3  'precipitant' 4  'dioxane'           5.0  .       'percent_volume_by_volume'

_pdbx_exptl_crystal_cryo_treatment.crystal_id   3

_pdbx_exptl_crystal_cryo_treatment.final_solution_details
; The cryoprotectant solution contained 30-35% (v/v) glycerol,
  40% saturated ammonium sulfate, 0.1M lactose/galactose in 0.1M
  glycine/HCl buffer at pH 2.5.
;

How this example will appear in the journal

Crystallization
Crystallization method Hanging-drop vapour diffusion  
Temperature Ambient
Crystallization solutions
   Protein (1 μl) Protein 10 mg ml-1, glycine/HCl 0.1 M, lactose/galactose 0.2 M, pH 2.5
   Precipitant (1 μl) ammonium sulfate 30-40% (w/v), glycine/HCl 0.1 M, lactose 0.1 M, dioxane 5.0% (w/v), pH 2.5
   Reservoir (0.79 ml) ammonium sulfate 30-40% (w/v), glycine/HCl 0.1 M, galactose 0.1 M, dioxane 5.0% (w/v), pH 2.5
Cryo treatment
  Final cryoprotection solution The cryoprotectant solution contained 30-35% (v/v) glycerol, 40% saturated ammonium sulfate, 0.1M lactose/galactose in 0.1M glycine/HCl buffer at pH 2.5



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