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Figure 4
Examples of SAXS patterns from frozen myofibrils, simultaneously recorded with the WAXS patterns in Fig. 3[link]. The reflections originate from the hexagonal lattice of myofilaments (see Fig. 1b[link]). (a) Sample frozen without a cryoprotectant; (b) sample frozen in the presence of 20% sucrose; (c) sample frozen in the presence of 20% MPD. The SAXS patterns cover the q-range up to 0.15 Å−1 here. One representative diffraction pattern is shown out of the 12 patterns recorded at each depth (the depth is indicated at the bottom right of each pattern). Whether a diffraction pattern shows a feature of a single hexagonal filament lattice [as in the 0 µm pattern in (b)] depends on the orientation and straightness of myofibrils in the muscle fiber, rather than the goodness of freezing. Note that, in 20% MPD, the reflection spots are finely defined even at 100 µm (c) while in 20% sucrose the reflection spots are blurred (b). The color scale is logarithmic.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 16| Part 3| March 2009| Pages 336-345
doi:10.1107/S0909049509004828
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