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Figure 4
(a) Size exclusion chromatography profile of α-crustacyanin. The absorbance is measured at two wavelengths, 280 nm (dashed line) and 630 nm (solid line). The major peak corresponding to purified α-crustacyanin is indicated with an asterisk. There is a smaller peak eluting later which corresponds to β-crustacyanin. (b) C(s) analysis of α-crustacyanin derived from sedimentation velocity AUC. α-Crustacyanin has a sedimentation coefficient of 11.4 ± 0.5 S, hydrodynamic radius of 6.8 nm and frictional ratio of 1.5. The experimental sedimentation coefficient is very similar to the theoretical value of 11.1 S for the EM-derived α-crustacyanin model.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 18| Part 1| November 2011| Pages 79-83
doi:10.1107/S0909049510034977
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