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Figure 4
Molecular functional group peak area ratio. Peak height under the CH3 asymmetric stretch at ∼2960 cm−1 divided by the height under the peaks of the CH2 asymmetric stretch at ∼2929 cm−1 at each pixel (pixel size 10 µm × 10 µm), representing the anti-symmetric CH3 to CH2 ratio in the sorghum seed tissue. (a) Visible image. (b) Spectra corresponding to the pixel at the cross hair in the visible image. (c) Chemical ratio image: anti-symmetric CH3 to CH2 ratio. (d) Region and baseline: anti-symmetric CH3 and CH2. (e) Three-dimensional image; peak height ratio of two peaks. (f) Ratio image profile set-up.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 18| Part 5| September 2011| Pages 790-801
https://doi.org/10.1107/S0909049511023727
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