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Figure 3
Root-mean-square error (RMSE) between predicted and measured gold particle positions using different similarity models. A total of 45 series were collected: for each series the RMSE is shown with a differently colored line as a function of frame number. Panel (a) shows the original RMSE, without registration. A drift correction was applied for the data shown in panel (b). Panel (c) shows a rigid movement registration in which translation, rotation and scale were refined, whereas panel (d) shows a full rigid movement registration where eight parameters were refined. The RMSE as a function of registration model is shown in panel (e).

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