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Figure 4
Microsecond irradiation of Alexa 488 solution. Dose response plot of 10 mM Alexa in 10 mM phosphate buffer, pH 7, using the 106 µm × 164 µm focused beam at ALS beamline 5.3.1. A 200 µL volume of Alexa solution was passed through the 100 µm ID capillary tube on the sample mount at different speeds (Table 1[link]) to obtain irradiation times in the range 15–120 µs. Fluorescence analysis was carried out as previously described (Gupta et al., 2007aBB20). The solid line represents a single exponential fit with a rate constant k = 13764 s−1, with individual points representing the mean of three independent measurements with standard error. The inset shows the dose-response plot of the same sample using a larger beam to irradiate samples homogeneously inside a 535 µm ID capillary tube, with an exponential fit rate constant of k = 6410 s−1.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 21| Part 4| May 2014| Pages 690-699
doi:10.1107/S1600577514007000
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