The following articles are a selection of those recently accepted for publication in Journal of Synchrotron Radiation.
Visualization of mouse spinal cord intramedullary arteries using phase- and attenuation-contrast tomographic imaging
Both phase- and attenuation-contrast tomographic imaging-based synchrotron radiation can be used to visualize the physiological arrangement of the entire intramedullary artery network in the mouse spinal cord at a high resolution level. Compared with attenuation-contrast imaging, phase-contrast imaging allows vessel visualization without contrast agent injection and is expected to be a reliable method for intramedullary artery detection.
Novel Silica Stabilization Method for the Analysis of Fine Nanocrystals Using Coherent X-ray Diffraction Imaging
In this study a novel silica stabilization method was designed by drop-casting a solution of tetraethylorthosilicate (TEOS) and ethanol onto nanocrystals (dried on a silicon substrate). Subsequent annealing encouraged polymerization of the silica embedding the nanocrystals into a silica matrix. BCDI investigations of the silica nanocomposites were performed at the Beamline 34-ID-C of the Advanced Photon Source, Argonne National Laboratory. three-dimensional diffraction data sets were collected from isolated FePt and AuPd nanocrystals. three-dimensional reconstructions of the nanocrystals morphology and strain field displacements were generated using a well established phasing algorithm. We demonstrate the feasibility of the novel silica stabilization method as a prompt and efficient approach to extend the accessible size regime of the BCDI technique. The success of this technique can also be seen by its rapid adoption by other APS beamline users (results as yet unpublished). In addition, this study reveals avenues for future exploration to optimize the stabilization technique by surface functionalization of the nanocrystals with a compatible surfactant. When used prior to stabilization, this coating could prevent agglomeration of the nanocrystals during annealing. Overall this BCDI study reveals the potential to probe smaller nanocrystals than previously achievable through the design of novel stabilization techniques. Furthering the knowledge and understanding of the fundamental properties of fine nanocrystals will enable key applications in medicine, catalysis and information technologies.
Guiding synchrotron X-ray diffraction by multimodal video-rate protein crystal imaging with synchronous digitization
Synchronous digitization, in which an optical sensor is probed synchronously with the firing of an ultrafast laser, was integrated into an optical imaging station for macromolecular crystal positioning prior to synchrotron X-ray diffraction. Using the synchronous digitization instrument, second-harmonic generation (SHG), two-photon excited fluorescence (TPEF), one photon excited fluorescence, two-photon excited ultraviolet fluorescence (TPE-UVF), and bright-field by laser transmittance were all acquired with perfect image registry at up to video-rate (15 frames per second).
Characterization of an X-ray mirror mechanical bender for the European XFEL
An X-ray mirror mechanical bender is characterized. The device is measured using a Fizeau large-aperture interferometer, and the most important effects, such as hysteresis, twisting, reproducibility and stability are investigated. The mirror silicon substrate is 950 mm long and the system is a possible candidate for the `adjustable' mirror element foreseen in the optical setup of the European XFEL beam transport.