2.4 Å resolution crystal structure of human TRAP1_NM, the Hsp90 paralog in the mitochondrial matrix

The 2.4 Å resolution crystal structure of human mitochondrial TRAP1_NM is reported, which together with epitope-mapping results supports a role for the M-domain in mediating the specific interaction with mitochondrial Hsp70 that is required for TRAP1-dependent protein folding.

Structural basis for the binding of succinate to succinyl-CoA synthetase

GTP-specific succinyl-CoA synthetase has been crystallized with CoA, magnesium and succinate to determine the binding site of the substrate succinate.

Crystal structure of the fluorescent protein from Dendronephthya sp. in both green and photoconverted red forms

The three-dimensional structure and the structure–function relationship of the photoconvertible fluorescent protein DendFP from Dendronephthya sp. are reported.

The 1.1 Å resolution structure of a periplasmic phosphate-binding protein from Stenotrophomonas maltophilia: a crystallization contaminant identified by molecular replacement using the entire Protein Data Bank

The structure of a periplasmic phosphate-binding protein from S. maltophilia has been determined at 1.1 Å resolution. The protein crystallized as an impurity, which was eventually identified by a PDB-wide molecular-replacement search followed by refinement at atomic resolution.

Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography

A sample-delivery system featuring high hit rates, low background and minimal sample requirements is presented for serial crystallography at XFELs and synchrotrons.

TakeTwo: an indexing algorithm suited to still images with known crystal parameters

A novel indexing method is presented that is well suited to the minimal information on a still image diffraction pattern and can achieve indexing rates of over one lattice per image.

RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments

An industrial six-axis robot has been combined with a high-accuracy air-bearing rotation axis to create a single device with the capabilities of both transferring cryocooled protein crystals from a sample-containing dewar and collecting complete X-ray diffraction data sets.

Structural basis for the extended substrate spectrum of AmpC BER and structure-guided discovery of the inhibition activity of citrate against the class C β-lactamases AmpC BER and CMY-10

The extended substrate spectrum conferred by the increased flexibility of the R2 loop and the inhibition activity of citrate towards the class C β-lactamases AmpC BER and CMY-10 is reported.