Welcoming five new Co-editors

Five new Co-editors are appointed to the Editorial Board of Acta Cryst. D – Structural Biology.

Advanced exploitation of unmerged reflection data during processing and refinement with autoPROC and BUSTER

The final models for macromolecular X-ray crystallography studies are usually not only the result of refinement against some version of scaled and merged reflection data, but are often also analysed and validated purely against such merged data. Here, various examples are presented to show how the availability and use of unmerged reflection data can lead to better model analysis and improved model parametrization, as well as providing a path to better data processing and scaling.

Using cryo-EM to understand the assembly pathway of respiratory complex I

Single-particle cryo-EM is a powerful technique to study the assembly process of the largest mitochondrial respiratory chain complex, NADH:ubiquinone oxidoreductase or complex I. Here, the new insights that have been gained into the molecular functions of assembly factors are reviewed.

A service-based approach to cryoEM facility processing pipelines at eBIC

The automation of cryoEM pipelines to aid data-quality analysis during acquisition poses a number of challenges, particularly at facilities where the expected data rates are high. The use of a modern service-based architecture deployed on Diamond Light Source's on-premises cloud computing cluster to tackle some of these challenges is demonstrated, focusing on the provision of an early-stage processing pipeline for electron tomography that produces first-attempt reconstructed volumes within minutes of tilt-series acquisition.

The crystal structure of myco­thiol di­sulfide reductase (Mtr) provides mechanistic insight into the specific low-molecular-weight thiol reductase activity of Actinobacteria

The crystal structure of mycothiol disulfide reductase (Mtr) was determined for the first time. The structure shows a highly conserved and enlarged substrate-binding pocket, providing insight into the substrate-binding mode and specificity of Mtrs.

XFEL structure of carbonic anhydrase II: a comparative study of XFEL, NMR, X-ray and neutron structures

An X-ray free-electron laser structure of human carbonic anhydrase II is presented at 1.7 Å resolution and compared with structures derived using other methods.

Characterization of novel mevalonate kinases from the tardigrade Ramazzottius varieornatus and the psychrophilic archaeon Methanococcoides burtonii

This work reports the purification, biochemical characterization and high-resolution structures of two novel mevalonate kinases: one from the extremotolerant tardigrade Ramazzottius varieornatus at 2 Å resolution and one from the psychrophilic archaeon Methanococcoides burtonii at 2.2 Å resolution.

Dynamics and Kinetics in Structural Biology: Unravelling Function Through Time-Resolved Structural Analysis