Interactive segmentation of membrane and membrane-mimic densities in cryo-EM maps

SURFER performs automated segmentation of contextual membrane and membrane-mimic density in cryo-EM maps to enable robust separation of macromolecular signal from surrounding detergent or lipid–membrane features. It is conveniently distributed as a plugin for UCSF ChimeraX, allowing interactive application within standard map-visualization workflows.

PETIMOT: a novel framework for inferring protein motions from sparse data using SE(3)-equivariant graph neural networks

We present a new formulation for protein flexibility and learn protein motions from sparse experimental data.

Experimental estimation of copper-site geometry reproducibility in biologically relevant redox and saccharide-bound states of a model lytic polysaccharide monooxygenase

Small but measurable changes in the coordination geometry of a lytic polysaccharide monooxygenase copper active site are revealed by X-ray crystallography, providing structural insight into their catalytic flexibility.

Dose-dependent structural and electron-density features in the lytic polysaccharide monooxygenase NcAA9D

A series of 36 structures of the Neurospora crassa LPMO NcAA9D were refined against X-ray crystal datasets collected with increasing dose from a single crystal to provide insights into dose-dependent structural changes at the active site of the enzyme. The findings underscore the importance of minimizing accumulated dose during cryo-X-ray crystallographic studies of lytic polysaccharide monooxygenases to prevent the misinterpretation of radiation damage-related electron-density features.

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM

We present a fully automated, unattended cryo-EM pipeline that serves as an on-the-fly diagnostic tool, enabling rapid data-quality assessment and informed decision-making to maximize data-collection efficiency.

Advances in the nanostructure characterization of biological hydrogels formed by the prion-like domain of EARLY FLOWERING 3

In biological systems, hydrogels often arise through liquid–liquid phase separation, where biomolecular condensates can age into gel-like states. SAXS is a key technique to probe the molecular organization of these samples. This study introduces an in-vacuum Gel-Cell for SAXS-based structural analysis of a biologically derived hydrogel formed by the thermosensory prion-like domain of EARLY FLOWERING 3 and presents a three-component model to interpret the obtained data.

Crystal structure of pilus-specific sortase from Enterococcus faecalis reveals both open and closed conformations: insights into loop dynamics and substrate recognition

The open and closed lid conformational states of class C sortase, which catalyzes the polymerization of endocarditis and biofilm-associated pili in E. faecalis, were captured in a single crystal. Communication between the flexible lid and the dynamic active-site (β7–β8) loop, along with their synchronized motions, regulates access to the active site for primary- and secondary-substrate binding.

Crystal structure of endo-β-1,6-galactanase from Streptomyces avermitilis

The crystal structure of S. avermitilis endo-β-1,6-galactanase belonging to GH30 subfamily 5 reveals the first structural framework for endo-β-1,6-galactanase. The β-1,6-galactobiose-bound complex identifies the catalytic subsites and a distal secondary sugar-binding site, providing insight into β-1,6-galactan recognition.

Structural basis for a p21-activated kinase 4 and nicotinamide phosphoribosyltransferase dual inhibitor

The 2-aminopyridine moiety of KPT-7523 binds p21-activated kinase 4 and nicotinamide phosphoribosyltransferase, enabling a versatile design for multi-target cancer therapy.

Protein Data Bank (PDB) Archive: a new architecture (beta) for scalable, PDBx/mmCIF-based data distribution

wwPDB has extended PDB IDs to 12 characters with a prefix `pdb_' followed by eight alphanumeric characters in lower case (for example pdb_1000axyz). A beta version of the PDB Archive (https://files-beta.wwpdb.org), with extended PDB IDs and an improved directory structure, is now available to help communities adopt extended PDB IDs and the PDBx/mmCIF format.

LowDoseWizard: rapid and standardized setup of low-dose cryo-TEM imaging in SerialEM

LowDoseWizard is a guided SerialEM workflow for rapid and standardized setup of low-dose cryo-TEM imaging conditions for single-particle analysis and cryo-electron tomography.

Replacement soaking for human tankyrase 2 enables studies on substrate analogues and inhibitors

Replacement soaking, also known as cross-soaking, is a compelling method for solving complex structures in cases where traditional soaking and co-crystallization approaches fail. This method is demonstrated with human tankyrase 2, and new crystal structures of complexes with nanomolar inhibitors and an analogue of the substrate NAD⁺ are reported.

Optimal 1TEL–target protein linker character is target protein-dependent. Corrigendum

The article by Pedroza Romo et al. [(2026), Acta Cryst. D82, 516–532] is corrected.

Structural basis for the fast maturation of pcStar, a photoconvertible fluorescent protein. Erratum

The article by Zheng et al. [(2025), Acta Cryst. D81, 181–195] is corrected.