Structural analysis of caspase-1 inhibitors derived from Tethering

The structure-based fragment-assembly approach described in the present study facilitated the rapid identification and subsequent synthesis of caspase-1 inhibitors with cell-based activity and provided insights into the enzyme's ability to accommodate different inhibitor-binding modes in the active site.

Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis

The crystal structure of purine nucleoside phosphorylase (DeoD) from B. anthracis was solved by X-ray crystallography using molecular replacement and refined at a resolution of 2.24 Å.

Structure of PIN-domain protein PH0500 from Pyrococcus horikoshii

The structure of P. horikoshii OT3 protein PH0500 was determined by the multiple anomalous dispersion method and refined in two crystal forms. The protein is a dimer and has a PIN-domain fold.

The structure at 2.4 Å resolution of the protein from gene locus At3g21360, a putative Fe^II/2-oxo­glutarate-dependent enzyme from Arabidopsis thaliana

The crystal structure of the 37.2 kDa At3g21360 gene product from A. thaliana was determined at 2.4 Å resolution. The structure establishes that this protein binds a metal ion and is a member of a clavaminate synthase-like superfamily in A. thaliana.

Crystallization and preliminary X-ray crystallographic studies of Mycobacterium tuberculosis chorismate mutase

Chorismate mutase from M. tuberculosis has been crystallized. Preliminary X-ray crystallographic studies reveal the occurrence of a dimeric molecule in the crystal asymmetric unit.

Crystallization and preliminary X-ray diffraction analysis of a chitin-binding domain of hyperthermophilic chitinase from Pyrococcus furiosus

The expression, purification and preliminary X-ray diffraction studies of a chitin-binding domain of the chitinase from P. furiosus are reported.

Crystallization and preliminary X-ray analysis of a novel thermoalkalophilic poly(3-hydroxybutyrate) depolymerase (PhaZ7) from Paucimonas lemoignei

A novel thermoalkalophilic depolymerase, PhaZ7, from P. lemoignei was crystallized by the microdialysis technique. Crystals belong to space group C2 and diffract to 2.75 Å resolution at a synchrotron source.

Crystallization and preliminary X-ray diffraction data for the aconitase form of human iron-regulatory protein 1

Two crystal forms of the aconitase version of recombinant human IRP1 are reported.

Crystallization and preliminary X-ray crystallographic analysis of yeast NAD⁺-specific isocitrate de­hydrogenase

Yeast NAD⁺-isocitrate dehydrogenase has been purified and crystallized using sodium citrate, a competitive inhibitor of the enzyme, as a precipitant. Preliminary X-ray analyses indicate the molecular boundaries of the molecule and large continuous solvent channels in the crystal.

Crystallization and preliminary X-ray analysis of (1R,6R)-2-succinyl-6-hy­droxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) synthase (MenD) from Escherichia coli

Single crystals of the holoenzyme (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase with ThDP and Mn²⁺ as cofactors were obtained by the hanging-drop vapour-diffusion method with 35% ethylene glycol as precipitant. Apoenzyme crystals were obtained by sitting-drop vapour diffusion with 70% MPD.

Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri

The first crystallographic study of a member of the YaeQ family of proteins, which are conserved in a small group of Gram-negative bacteria, most of which are animal or plant pathogens, is reported. Diffraction data were collected to 1.9 Å resolution and an interpretable electron-density map was obtained.

Preparation, crystallization and preliminary X-ray analysis of YjcG protein from Bacillus subtilis

B. subtilis YjcG protein was expressed, purified and crystallized. A complete diffraction data set was collected at BSRF beamline 3W1A and processed to 2.3 Å resolution.

Recombinant bovine uteroglobin at 1.6 Å resolution: a preliminary X-ray crystallographic analysis

The crystallization of recombinant bovine uteroglobin.

Crystallization and preliminary X-ray characterization of a ferritin from the hyperthermophilic archaeon and anaerobe Pyrococcus furiosus

Ferritin from P. furiosus crystallizes in space group C222₁, with unit-cell parameters a = 258.1, b = 340.1, c = 266.5 Å and 36 monomers in the asymmetric unit, corresponding to one and a half 24-mers.

Purification, crystallization and preliminary X-ray diffraction studies of the archaeal virus resolvase SIRV2

The Holliday junction resolvase of the archaeal virus SIRV2 infecting the archaeon Sulfolobus islandicus has been crystallized and a full data set has been collected at 3.4 Å resolution. Analysis of the self-rotation function suggests the presence of two dimers in the asymmetric unit with a solvent content of 77%.

Crystallization and preliminary X-ray studies of mouse centrin1

The expression, purification, crystallization and preliminary X-ray diffraction studies of mouse centrin1 are reported.

Crystallization and preliminary X-ray analysis of mitochondrial presequence receptor Tom20 in complexes with a presequence from aldehyde de­hydrogenase

A presequence peptide derived from rat aldehyde dehydrogenase was tethered to the cytosolic domain of rat Tom20 protein via an intermolecular disulfide bond. Two crystal forms were obtained with different linker designs and diffracted to 2.1 and 1.9 Å.

Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis

Mycobacterial PimA is an essential enzyme that catalyses the first mannosylation step in phosphatidyl-myo-inositol mannoside (PIM) biosynthesis. Crystals of the enzyme from M. smegmatis, obtained in the presence of GDP and myo-inositol, are orthorhombic (P2₁2₁2₁) and diffract X-rays to 2.4 Å resolution.

Purification, crystallization and preliminary X-ray diffraction analysis of human enolase-phosphatase E1

Recombinant human E1 enzyme has been crystallized using the hanging-drop vapour-diffusion method and diffraction-quality crystals were grown at 291 K using PEG 4000 as precipitant.

Purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1)

Two different fragments of the ligand-binding domain of LOX-1, the major receptor for oxidized low-density lipoprotein (LDL) on endothelial cells, have been crystallized in different forms.

Crystallization and preliminary crystallographic analysis of a flavoprotein NADH oxidase from Lactobacillus brevis

The water-forming flavoenzyme NADH oxidase was crystallized successfully for the first time. The crystals diffract X-rays to at least 4.0 Å resolution.

Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human transcription repressor ERH

Crystallization of human enhancer of rudimentary homologue protein.

Initiating a crystallographic analysis of recombinant (S)-2-hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis

The gene encoding the unusual metal-ion-dependent epoxidase involved in fosfomycin biosynthesis, S. wedmorensis (S)-2-hydroxypropylphosphonic acid epoxidase, has been cloned and the protein expressed, purified and crystallized. Two crystal forms have been obtained, one of which diffracts to high resolution.

Isolation, crystallization and preliminary X-ray analysis of a methanol-induced corrinoid protein from Moorella thermo­acetica

Strongly diffracting crystals of a methanol-induced corrinoid protein from M. thermoacetica have been obtained.