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Structure of the synthetase domain of human CTP synthetase, a target for anticancer therapy

The crystal structure of the synthetase domain of human CTPS has been determined. The structure is the first of an eukaryotic CTPS and provides a starting point for drug design towards CTPS.

X-ray crystallographic studies reveal that the incorporation of spacer groups in carbonic anhydrase inhibitors causes alternate binding modes

The crystal structure of human CA II has been determined in complex with two CA inhibitors (CAIs) containing conventional sulfonamide and thiadiazole moieties separated by a —CF₂— or —­CHNH₂— spacer group.

Tris(hydroxymethyl)amino­methane induces conformational change and crystal-packing contraction of porcine pancreatic elastase

The structure of porcine pancreatic elastase at 1.5 Å resolution has a unique conformation induced by tris(hydroxymethyl)aminomethane as a buffer component.

Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7

Crystals of Stx2 were grown in the presence of adenosine and adenine. In both cases, the resulting electron density showed only adenine bound at the active site of the A subunit, proving that the holotoxin is an active N-glycosidase.

Structure of human MIP-3α chemokine

Human macrophage inflammatory protein-3α (MIP-3α) has been crystallized in a new space group I4. Crystals diffract to 1.81 Å.

Cloning, expression, purification and preliminary crystallographic characterization of a shikimate de­hydrogenase from Corynebacterium glutamicum

The crystallization and preliminary X-ray characterization of a shikimate dehydrogenase from C. glutamicum is presented.

Crystallization and preliminary X-ray crystallographic analysis of the Escherichia coli outer membrane cobalamin transporter BtuB in complex with the carb­oxy-terminal domain of TonB

Crystals of a complex of the E. coli proteins BtuB (outer membrane cobalamin transporter) and TonB (carboxy-terminal domain) diffracting to 2.1 Å resolution have been obtained.

Crystallization and preliminary X-ray analysis of a Kunitz-type inhibitor, textilinin-1 from Pseudonaja textilis textilis

Crystals of a canonical inhibitor of plasmin from Australian Brown snake venom has been obtained. In complex with trypsin these diffract to 2.0 Å resolution, while the free inhibitor diffracts to 1.63 Å.

Crystallization and preliminary crystallographic analysis of the family GH78 α-L-rhamnosidase RhaB from Bacillus sp. GL1

The crystallization and preliminary crystallographic analysis of a family GH78 α-L-rhamnosidase, RhaB from Bacillus sp. GL1, are presented.

Crystallization and preliminary X-ray diffraction data of the complex between human centrin 2 and a peptide from the protein XPC

Production, crystallization and phasing procedures are reported for the complex of human centrin 2, lacking the first 25 residues, and a 17-residue peptide from the protein XPC.

Overexpression, purification and preliminary crystallographic analysis of human M-ficolin fibrinogen-like domain

Human M-ficolin fibrinogen-like domain has been overexpressed in P. pastoris, purified and crystallized. Diffraction data have been collected to 1.9 Å.

Crystallization and preliminary X-ray analysis of the PIN domain of human EST1A

The PIN domain of human EST1A was expressed, purified and crystallized by the sitting-drop vapour-diffusion method.

The purification, crystallization and preliminary diffraction of a glycero­phosphodiesterase from Enterobacter aerogenes

The metallo-glycerophosphodiesterase from E. aerogenes (GpdQ) has been cloned, expressed in E. coli and purified. Initial screening of crystallization conditions for this enzyme resulted in the identification of needles from one condition in a sodium malonate grid screen. Removal of the metals from the enzyme and subsequent optimization of these conditions led to crystals.

Crystallization and preliminary X-ray diffraction studies of ferredoxin reductase from Leptospira interrogans

Crystals adequate for X-ray diffraction analysis have been prepared from L. interrogans ferredoxin-NADP⁺ reductase.

Comparative crystallization and preliminary X-ray diffraction studies of locked nucleic acid and RNA stems of a tenascin C-binding aptamer

Locked nucleic acid (LNA) nucleotides are RNA analogues with a useful additional conformational constraint; the current investigation will provide the first crystallographic view of an all-LNA duplex.

Crystallization and preliminary X-ray diffraction analysis of a protease inhibitor from the haemolymph of the Indian tasar silkworm Antheraea mylitta

The crystallization and preliminary X-ray crystallographic analysis of a protease inhibitor from the haemolymph of the Indian tasar silk worm A. mylitta is reported.

Overexpression, purification and crystallization of a choline-binding protein CbpI from Streptococcus pneumoniae

The choline-binding protein CbpI from S. pneumoniae has been purified and crystallized and diffraction data have been collected to 3.5 Å resolution.

Purification, crystallization and preliminary X-ray diffraction analysis of RafE, a sugar-binding lipoprotein from Streptococcus pneumoniae

The mature form of RafE has been expressed, purified and crystallized. X-ray diffraction data have been collected to 3.65 and 2.90 Å resolution from native and selenomethionine-derivative crystals, respectively.

Crystallization and preliminary X-ray diffraction analysis of an anti-H(O) lectin from Lotus tetragonolobus seeds

The seed lectin from Lotus tetragonolobus (LTA) has been crystallized. The best crystals grew over several days and were obtained using the vapour-diffusion method at a constant temperature of 293 K.

Expression, purification, crystallization and preliminary diffraction data characterization of Escherichia coli ribonuclease II (RNase II)

Diffraction data from E. coli RNase II crystals of wild type and of an inactive mutant and its SeMet-derivative form were obtained to 2.44 and 2.74 Å resolution, providing a set of preliminary phases. An improved purification protocol allowed higher reproducibility in the crystallization of the mutant form.

Crystallization and preliminary X-ray crystallographic analysis of two vascular apoptosis-inducing proteins (VAPs) from Crotalus atrox venom

Vascular apoptosis-inducing protein 1 (VAP1) and VAP2 from C. atrox venom were crystallized in variety of different crystal forms. Diffraction data sets were obtained to 2.5 and 2.15 Å resolution for VAP1 and VAP2, respectively.

Purification, crystallization and preliminary X-ray diffraction studies of Rab11 in complex with Rab11-FIP2

Crystals of Rab11 in complex with the Rab-binding domain (RBD) of its effector Rab11-FIP2 were grown and synchrotron diffraction data were collected at 2.8 Å resolution.

Cloning, overexpression, purification and preliminary crystallographic studies of a mitochondrial type II peroxiredoxin from Pisum sativum

The isolation, purification, crystallization and molecular-replacement solution of mitochondrial type II peroxiredoxin from P. sativum is reported.

Crystallization and preliminary X-ray characterization of aminopeptidase N from Escherichia coli

E. coli aminopeptidase N has been crystallized by the vapour-diffusion method. Diffraction data have been collected and processed to 2.0 Å resolution.

Crystallization of DIR1, a LTP2-like resistance signalling protein from Arabidopsis thaliana

DIR1, a putative LTP2 protein from Arabidopsis thaliana implicated in systemic acquired resistance in planta, has been crystallized in space group P2₁2₁2₁ with one molecule per asymmetric unit.

Cloning, purification and crystallization of Bacillus anthracis class C acid phosphatase

Crystallization of a surface-localized acid phosphatase from Bacillus anthracis is reported. Flash annealing increased the high-resolution limit of usable data from 1.8 to 1.6 Å.

Crystallization and preliminary X-ray crystallographic analysis of yeast NAD⁺-specific isocitrate de­hydrogenase. Corrigendum

A corrigendum to the article by Hu et al. (2005), Acta Cryst. F61, 486–488.