The structures of pyruvate oxidase from Aerococcus viridans with cofactors and with a reaction intermediate reveal the flexibility of the active-site tunnel for catalysis

The crystal structures of pyruvate oxidase from A. viridans in complex with flavin adenine dinucleotide, thiamine diphosphate and the reaction intermediate 2-acetyl-thiamine diphosphate reveal details of substrate recognition and catalysis.

Structure of Staphylococcus aureus 1,4-di­hydroxy-2-naphthoyl-CoA synthase (MenB) in complex with aceto­acetyl-CoA

The structure of S. aureus MenB, an enzyme in the biosynthetic pathway to vitamin K₂, has been determined and compared with the enzyme derived from another important pathogen, M. tuberculosis.

A high-resolution structure of the DNA-binding domain of AhrC, the arginine repressor/activator protein from Bacillus subtilis

The structure of the winged helix–turn–helix DNA-binding domain of AhrC has been determined at 1.0 Å resolution. The largely hydrophobic β-wing shows high B factors and may mediate the dimer interface in operator complexes.

Structure of the C-terminal effector-binding domain of AhrC bound to its corepressor L-arginine

The crystal structure of the C-terminal domain hexameric core of AhrC, with bound corepressor (L-arginine), has been solved at 1.95 Å resolution. Binding of L-arginine results in a rotation between the two trimers of the hexamer, leading to the activation of the DNA-binding state.

Structure of the ribosomal interacting GTPase YjeQ from the enterobacterial species Salmonella typhimurium

The X-ray crystal structure of the GTPase YjeQ from S. typhimurium is presented and compared with those of orthologues from T. maritima and B. subtilis.

Crystallization and preliminary crystallographic studies of Schizolobium parahyba chymotrypsin inhibitor (SPCI) at 1.8 Å resolution

Crystallization and preliminary crystallographic studies of Schizolobium parahyba chymotrypsin inhibitor (SPCI) at 1.8 Å resolution.

Purification, crystallization and preliminary X-ray analysis of a thermostable glycoside hydrolase family 43 β-xylosidase from Geobacillus thermoleovorans IT-08

The β-xylosidase was crystallized using PEG 6000 as precipitant. 5% PEG 6000 yielded bipyramid-shaped tetragonal crystals diffracting to 1.55 Å resolution, and 13% PEG 6000 gave rectangular monoclinic crystals diffracting to 1.80 Å resolution.

Expression, purification and preliminary X-ray analysis of the C-terminal domain of an arginine repressor protein from Mycobacterium tuberculosis

The C-terminal portion of the arginine repressor protein from M. tuberculosis H37Rv has been crystallized. The complete transcriptional factor regulates arginine biosynthesis by binding operator DNA when arginine is bound at the C-terminal domain.

Expression, purification, crystallization and preliminary X-ray analysis of the Met244Ala variant of catalase–peroxidase (KatG) from the haloarchaeon Haloarcula marismortui

The Met244Ala variant of the H. marismortui KatG enzyme was expressed in haloarchaeal host cells and purified to homogeneity. The variant was crystallized using the hanging-drop vapour-diffusion method with ammonium sulfate and NaCl as precipitants. The reddish-brown rod-shaped crystals obtained belong to the monoclinic space group C2, with unit-cell parameters a = 315.24, b = 81.04, c = 74.77 Å, β = 99.81°.

Expression, purification, crystallization and initial crystallographic characterization of the p-hydroxybenzoate hydroxylase from Corynebacterium glutamicum

PHBH from Corynebacterium glutamicum was crystallized using the hanging-drop vapour-diffusion method in the presence of NaH₂PO₄ and K₂HPO₄ as precipitants. X-ray diffraction data were collected to a maximum resolution of 2.5 Å on a synchrotron beamline.

Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase

Octaketide synthase from A. arborescens has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 2.6 Å.

Purification, crystallization and preliminary X-ray diffraction study of human ribosomal protein L10 core domain

A truncated variant of human ribosomal protien L10 was prepared and crystallized. Diffraction data were collected to 2.5 Å resolution.

Expression and crystallization of DsbA from Staphylococcus aureus

Free-interface diffusion crystallization chips were used to identify crystallization conditions for S. aureus DsbA, representing the first Gram-positive DsbA to be crystallized. Native and selenomethionine-derivative crystals diffracted to 2.1 and 2.4 Å resolution, respectively.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of universal stress protein F (YnaF) from Salmonella typhimurium

The cloning, purification and crystallization of YnaF from S. typhimurium are reported along with preliminary X-ray crystallographic studies.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2)

Chloride intracellular channel 2 (CLIC2) belongs to a family of intracellular chloride-channel proteins that can exist in a soluble form. The expression, purification and crystallization in two different crystal forms of human CLIC2 is reported.

Purification, crystallization and preliminary X-ray crystallographic analysis of ST1022, a putative member of the Lrp/AsnC family of transcriptional regulators isolated from Sulfolobus tokodaii strain 7

A putative member of the Lrp/AsnC family of transcriptional regulators, ST1022 from S. tokodaii strain 7, has been purified and crystallized in the absence and presence of the effector L-glutamine. A molecular-replacement solution was found using the FL11 transcriptional regulator from Pyrococcus sp. OT3 as a model and structural refinement is under way.

Crystallization and preliminary crystallographic analysis of recombinant human galectin-1

Human galectin-1 has been cloned, expressed in E. coli, purified and crystallized in the presence of both lactose (ligand) and β-mercaptoethanol under six different conditions. The X-ray diffraction data obtained have enabled the assignment of unit-cell parameters for two novel crystal forms of human galectin-1.

Improving protein crystal quality by selective removal of a Ca²⁺-dependent membrane-insertion loop

Protein engineering dramatically enhances the quality of crystals of a Ca²⁺-dependent membrane-binding protein.

Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2

The crystallization of the brazil nut allergen Ber e 2 is reported.

Expression, purification and preliminary X-ray diffraction studies of RebC

The flavin-dependent monooxygenase RebC has been crystallized by the hanging-drop vapour-diffusion method and initial X-ray diffraction analysis has been completed.

Crystallization and preliminary X-ray diffraction studies of tetrameric malate de­hydrogenase from the novel Antarctic psychrophile Flavobacterium frigidimaris KUC-1

A psychrophilic malate dehydrogenase from the novel Antarctic bacterium F. frigidimaris KUC-1 was crystallized using the hanging-drop vapour-diffusion method. The crystals contained one tetrameric molecule per asymmetric unit. The best crystal diffracted to 1.8 Å resolution.

Purification, crystallization and preliminary X-ray analysis of stru­thiocalcin 1 from ostrich (Stru­thio camelus) eggshell

The purification, crystallization and preliminary X-ray diffraction data of the protein struthiocalcin 1 isolated from ostrich eggshell are reported.

Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase

Preliminary X-ray diffraction studies of apo maize aldose reductase at 2.0 Å resolution are reported.