Deposition of diffraction images to be discussed at the Open Meeting of the Commission on Biological Macromolecules of the IUCr in Osaka

Structural analysis of the complex of Keap1 with a prothymosin α peptide

The crystal structure of the complex of mouse Keap1-DC with a fragment of the nuclear protein prothymosin α was determined and refined to 1.9 Å resolution and revealed that the peptide binds to the bottom region of the β-propeller domain of Keap1-DC.

Monoclinic form of isopentenyl diphosphate isomerase: a case of polymorphism in biomolecular crystals

Type 1 isopentenyl diphosphate isomerase (IDI-1) in a new crystal form.

Structure of the SH3 domain of rat endophilin A2

The crystal structure of the SH3 domain of rat endophilin A2 has been determined by the multiwavelength anomalous dispersion method and refined at a resolution of 1.70 Å to R and R_free values of 0.196 and 0.217, respectively.

Structure of the cold-shock domain protein from Neisseria meningitidis reveals a strand-exchanged dimer

The X-ray crystal structure of the cold-shock domain protein from N. meningitidis reveals a strand-exchanged dimer.

Crystallization and preliminary X-ray crystallographic analysis of a carbonyl reductase from Candida parapsilosis

A novel short-chain NADPH-dependent (S)-1-phenyl-1,2-ethanediol dehydrogenase (SCR) has been crystallized.

Crystallization and preliminary X-ray diffraction analysis of the glucuronoyl esterase catalytic domain from Hypocrea jecorina

The catalytic domain of the glucuronoyl esterase from H. jecorina was overexpresssed, purified and crystallized in space group P2₁2₁2₁. X-ray diffraction data were collected to 1.9 Å resolution.

Expression, purification and crystallization of a lyssavirus matrix (M) protein

The expression, purification and crystallization of the full-length matrix protein from three lyssaviruses is described.

Structural analysis of bacteriophage-encoded peptidoglycan hydrolase domain KMV36C: crystallization and preliminary X-ray diffraction

Crystallization and X-ray data collection of the C-terminus of gp36 from bacteriophage φKMV (KMV36C) are reported.

Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to the extracellular IL-22R1 chain

Crystals of the IL-22–sIL-22R1 complex have been obtained by hanging-drop vapor-diffusion experiments combined with streak-seeding at 298 K. A complete low-temperature 3.2 Å resolution data set has been collected using synchrotron radiation.

Preliminary time-of-flight neutron diffraction study of human deoxyhemoglobin

In order to investigate the role of the protonation states of protein residues in O₂ binding, large crystals of deoxy HbA (∼20 mm³) were grown in D₂O under anaerobic conditions for neutron diffraction studies.

Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis

The second GAF domain of the hypoxia-sensing DevS from M. smegmatis has been crystallized. The crystal belonged to space group P6₁ and diffracted to 2.0 Å resolution.

Cloning, purification and preliminary X-ray analysis of the C-terminal domain of Helicobacter pylori MotB

The cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of a putative peptidoglycan-binding domain of H. pylori MotB, a stator component of the bacterial flagellar motor, are reported.

Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum

The cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from T. flavum, a protein which catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, are reported.

Crystallization and preliminary crystallographic analysis of the transpeptidase domain of penicillin-binding protein 2B from Streptococcus pneumoniae

The selenomethionyl-substituted transpeptidase domain of penicillin-binding protein (PBP) 2B from S. pneumoniae was isolated from a limited proteolysis digest of the soluble form of recombinant PBP 2B and then crystallized. MAD data were collected to 2.4 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of the multidrug efflux transporter NorM from Neisseria gonorrhoeae

The multidrug efflux transporter NorM from N. gonorrhoeae has been crystallized and X-ray diffraction data have been collected to a resolution of 6.5 Å.

Crystallization and preliminary X-ray diffraction studies of Seneca Valley Virus-001, a new member of the Picornaviridae family

Seneca Valley Virus-001 of the Picornavirdae family was crystallized in the space group R3 and X-ray diffraction data was collected to a resolution of 2.3 Å. Rotation-function studies suggested the presence of two distict sets of 20 protomers that belong to two different virus particles in the crystallographic asymmetric unit.

Crystallization of an Lhx3–Isl1 complex

An intramolecular complex comprising the LIM domains of Lhx3 and the interacting domain of Isl1 tethered by a flexible linker was engineered, overexpressed in E. coli, purified and crystallized.

Preliminary structural studies on the MtxX protein from Methanococcus jannaschii

In this study, the MtxX protein from M. jannaschii has been cloned, expressed, purified and crystallized.

Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone

Benzalacetone synthase from R. palmatum has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 1.8 Å.

Crystallization and preliminary X-ray analysis of the complex of the first von Willebrand type C domain bound to bone morphogenetic protein 2

Crystals of the complex of the first von Willebrand type C domain (VWC1) of crossveinless 2 (CV2) bound to bone morphogenetic protein 2 (BMP2) exist in two tetragonal crystal forms belonging to either space group P4₁2₁2 or I4₁, with one complete BMP2 dimer and two CV2 VWC1 domains per asymmetric unit, and diffract to 2.6 Å resolution.

Crystallization of hepatocyte nuclear factor 4α (HNF4α) in complex with the HNF1α promoter element

Sample preparation, characterization, crystallization and preliminary X-ray analysis are reported for the HNF4α–DNA binary complex.

Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis E virus-like particle

A recombinant virus-like particle that is a potential oral hepatitis E vaccine was crystallized. Diffraction data were collected to 8.3 Å resolution and the X-ray structure was phased with the aid of a low-resolution density map determined using cryo-electron microscopy data.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of thioredoxin Trx1 from Saccharomyces cerevisiae

In the present work, the cytosolic form of thioredoxin from S. cerevisiae was cloned, expressed, purified and crystallized.

Protein Condensation