Structure of the catalytic domain of Streptococcus pneumoniae sialidase NanA

The structure of a catalytically active subdomain of the NanA sialidase from S. pneumoniae is reported to a resolution of 2.5 Å. The complex with the inhibitor Neu5Ac2en identifies the key catalytic residues and provides a platform for structure-based development of specific inhibitors.

Structure of the catalytic trimer of Methanococcus jannaschii aspartate transcarbamoylase in an orthorhombic crystal form

The structure of the catalytic subunit of M. jannaschii aspartate transcarbamoylase has been determined in space group P2₁2₁2₁ using synchrotron data to a resolution of 3.0 Å and was refined to a final R_work and R_free of 0.215 and 0.269, respectively.

Purification, crystallization and preliminary X-ray analysis of human mannose-binding lectin-associated serine protease-1 (MASP-1) catalytic region

Acidic nondenaturing PAGE revealed inhomogeneity of the MASP-1 catalytic region caused by deamidation as the reason behind previous unsuccessful crystallization attempts. Monitoring the separation of the various species by acidic nondenaturing PAGE during purification helped to obtain pure protein, which was subsequently crystallized.

Expression, purification, crystallization and preliminary X-ray studies of histamine de­hydrogenase from Nocardioides simplex

Histamine dehydrogenase from Nocardioides simplex has been expressed, purified and crystallized with full incorporation of 6-S-cysteinyl-FMN. Diffraction data have been collected to 2.7 Å resolution; the crystals belonged to the orthorhombic space group P2₁2₁2₁.

Expression, purification, crystallization and preliminary X-ray studies of a prolyl-4-hydroxylase protein from Bacillus anthracis

Prolyl-4-hydroxylase from B. anthracis has been cloned, expressed and crystallized. A complete MAD data set has been collected to 1.4 Å resolution.

Preliminary X-ray diffraction analysis of YqjH from Escherichia coli: a putative cytoplasmic ferri-siderophore reductase

Crystallization of the proposed FAD-containing ferri-siderophore reductase protein YqjH from E. coli has been performed and the structure has been determined to 3.0 Å resolution. The structure shows similarity to another proposed siderophore-interacting protein from S. putrefaciens and weak similarity to members of the NAD(P)H:flavin oxidoreductase superfamily.

Crystallization and preliminary X-ray analysis of CrgA, a LysR-type transcriptional regulator from pathogenic Neisseria meningitidis MC58

The full length and the regulatory domain of the LysR-type transcriptional regulator CrgA have been crystallized. Diffraction data were collected from two crystal forms of full-length CrgA to 3.0 and 3.8 Å resolution, respectively. Crystals of the selenomethionine derivative of the C-terminal regulatory domain of CrgA diffracted to 2.3 Å resolution.

Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain

The crystal of the nsp3 ADRP domain of avian infectious bronchitis virus (IBV) has been obtained and subjected to further crystallograghic studies.

Expression, purification and preliminary crystallographic analysis of N-acetylglucosamine-1-phosphate uridylyltransferase from Mycobacterium tuberculosis

N-Acetylglucosamine 1-phosphate uridyltransferase (GlmU) from M. tuberculosis H37Rv has been crystallized and preliminary X-ray crystallographic analysis has been performed. GlmU is a bi-domained bifunctional enzyme that is involved in the biosynthesis of UDP-N-acetylglucosamine, a precursor in peptidoglycan biosynthesis in M. tuberculosis.

Crystallization and preliminary X-ray diffraction analysis of the peptidylprolyl isomerase Par27 of Bordetella pertussis

Par27 from B. pertussis, the prototype of a new group of parvulins has been crystallized in two different crystal forms.

Crystallization and preliminary crystallographic analysis of Gibberella zeae extracellular lipase

G. zeae extracellular lipase has been overexpressed, purified and crystallized. Diffraction data were collected to 2.8 Å resolution.

Crystallization and preliminary X-ray diffraction studies of the calcium-binding protein CalD from Streptomyces coelicolor

The calcium-binding protein encoded by the CalD gene in S. coelicolor A3(2) has been crystallized as a prelude towards the determination of its three-dimensional structure by X-ray crystallography.

Crystallographic study of G178S mutant of human proliferating cell nuclear antigen

Crystallization and diffraction studies of human PCNA G178S mutant are reported.

Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol δ

The cloning, expression, purification and crystallization of the complex of the second and third regulatory subunits of human Pol δ are reported. The crystals were characterized and an X-ray diffraction data set was collected to a resolution of 3 Å.

Purification, crystallization and preliminary X-ray diffraction analysis of a cysta­thionine β-synthase domain-containing protein, CDCP2, from Arabidopsis thaliana

Native and selenomethionine-derivatized CDCP2 from A. thaliana have been overexpressed, purified and crystallized. The crystals diffracted to 2.4 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of L-threonine de­hydrogenase (TDH) from the hyperthermophilic archaeon Thermococcus kodakaraensis

The L-threonine dehydrogenase from T. kodakaraensis KOD1 has been crystallized in the tetragonal space group P4₃2₁2, with unit-cell parameters a = b = 124.5, c = 271.1 Å. Diffraction data were collected to 2.6 Å resolution and preliminary analysis indicates that there are four molecules in the crystallographic asymmetric unit.

Purification and crystallization of a non-GluR2 AMPA-receptor ligand-binding domain: a case of cryo-incompatibility addressed by room-temperature data collection

High-resolution diffraction was obtained from GluR4 AMPA-receptor ligand-binding domain crystals using a combination of cryogenic and room-temperature data-collection strategies.

Coenzyme- and His-tag-induced crystallization of octopine de­hydrogenase

The crystal structure of octopine dehydrogenase revealed a specific role of the His₅ tag in inducing the crystal contacts required for successful crystallization.

Cloning, expression, purification, crystallization and preliminary X-ray analysis of the human RuvBL1–RuvBL2 complex

A truncated variant of the human RuvBL1–RuvBL2 complex was cloned, expressed, purified and crystallised. Synchrotron diffraction data to 4 Å resolution were used to carry out a preliminary crystallographic analysis of the complex.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of hypothetical protein SCO4226 from Streptomyces coelicolor A3(2)

A hypothetical protein SCO4226 from S. coelicolor has been overexpressed, purified and crystallized. The crystals belonged to space group P2₁ and diffracted to 2.0 Å resolution.

Crystallization and preliminary X-ray crystallographic study of flavoredoxin from Desulfovibrio vulgaris Miyazaki F

Flavoredoxin from D. vulgaris Miyazaki F has been purified and crystallized. The crystals diffracted to 1.05 Å resolution using synchrotron radiation.

Crystallization and X-ray analysis of the Schistosoma mansoni guanidino kinase

The X-ray structure of the guanidino kinase from S. mansoni has been determined at 2.8 Å resolution by the molecular-replacement method.

Screening of detergents for solubilization, purification and crystallization of membrane proteins: a case study on succinate:ubi­quinone oxidoreductase from Escherichia coli

Succinate:ubiquinone oxidoreductase was solubilized and purified from E. coli inner membranes using several different detergents and the phospholipid content of each preparation was analyzed. The preparation with the lowest phospholipid content crystallized in two different crystal forms using detergent mixtures composed of n-alkyl-oligoethylene glycol monoether and n-alkyl-maltoside.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of tetra­hydrodipicolinate-N-succinyltransferase (Rv1201c) from Mycobacterium tuberculosis

M. tuberculosis tetrahydrodipicolinate-N-succinyltransferase, the enzyme that catalyses the fifth reaction step of the lysine-biosynthesis pathway, has been cloned, expressed, purified and crystallized.

Crystallographic characterization of the N-terminal domain of a plant NADPH oxidase

A crystal of the N-terminal domain of a plant NADPH oxidase was obtained and X-ray diffraction data were collected on a synchrotron beamline to a maximum resolution of 2.4 Å.

Rational `correction' of the amino-acid sequence of RbcX protein from the thermophilic cyanobacterium Thermosynechococcus elongatus dramatically improves crystallization

Recombinant RuBisCO chaperone from T. elongatus, TeRbcX, was aggregated and could not be crystallized. Mutations of an unusual Cys residue in the TeRbcX sequence yielded much better behaved proteins that could rapidly be crystallized.