Streamlining in 2008

The high-resolution structure of di­hydrodipicolinate synthase from Escherichia coli bound to its first substrate, pyruvate

The structure of E. coli dihydrodipicolinate synthase cocrystallized with oxaloacetic acid has been solved at a resolution of 2.0 Å. The structure was found to contain a pyruvate molecule bound at the active site rather than oxaloacetate.

1.9 Å structure of the signal receiver domain of the putative response regulator NarL from Mycobacterium tuberculosis

The crystal structure of the signal receiver domain of NarL from M. tuberculosis, a putative nitrate response regulator involved in the regulation of anaerobic metabolism, has been determined to 1.9 Å resolution.

Structure of the wild-type human BCL6 POZ domain

The crystal structure of the wild-type BCL6 POZ domain confirms the structure of previously reported mutant proteins and will have relevance for the design of therapeutics that target BCL6 POZ-domain function.

Structure of a xenon derivative of Escherichia coli copper amine oxidase: confirmation of the proposed oxygen-entry pathway

The structure of a xenon derivative of E. coli copper amine oxidase confirms the pathway of oxygen entry to the buried active site proposed for this class of enzymes.

Purification, crystallization and X-ray structures of the two manganese superoxide dismutases from Caenorhabditis elegans

Two manganese superoxide dismutase enzymes isolated from the eukaryote C. elegans have been characterized and their structures determined. The closely related structures reveal a striking similarity to manganese superoxide dismutase found in humans.

Expression, crystallization and preliminary X-ray crystallographic analysis of Xoo0352, D-alanine-D-alanine ligase A, from Xanthomonas oryzae pv. oryzae

Xoo0352, which encodes D-alanine-D-alanine ligase A (DdlA), from X. oryzae pv. oryzae was cloned, purified and crystallized. Preliminary X-ray crystallographic analysis of DdlA crystals was performed.

Crystallization and preliminary X-ray diffraction analysis of phospholipase A₁ isolated from hornet (Vespa basalis) venom

Crystals of phospholipase A₁ from hornet (V. basalis) venom were obtained that diffracted X-rays to a resolution of at least 2.5 Å resolution.

Preliminary crystallographic analysis of GpgS, a key glu­cosyltransferase involved in methylglucose lipopolysaccharide biosynthesis in Mycobacterium tuberculosis

Glucosyl-3-phosphoglycerate synthase (GpgS) is a key enzyme that catalyses the first glucosylation step in methylglucose lipopolysaccharide biosynthesis in Mycobacterium spp. Here, the crystallization and preliminary crystallographic analysis of GpgS from M. tuberculosis and of its complex with UDP are reported.

Crystallization and preliminary X-ray crystallographic analysis of Ca²⁺-free primary Ca²⁺-sensor of Na⁺/Ca²⁺ exchanger

The plasma-membrane Na⁺/Ca²⁺ exchanger (NCX) regulates intracellular Ca²⁺ levels in cardiac myocytes. Two Ca²⁺-binding domains (CBD1 and CBD2) exist in the large cytosolic loop of NCX. Recombinant CBD1 (NCX1 372–508) with a molecular weight of 16 kDa has been crystallized by the sitting-drop vapour-diffusion method at 293 K.

Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of 3-de­hydroquinate synthase, Xoo1243, from Xanthomonas oryzae pv. oryzae

Bacterial blight is a destructive disease of rice that is caused by X. oryzae pv. oryzae (Xoo). Dehydroquinate synthase, which is the second enzyme of the shikimate pathway, was cloned from Xoo1243 (aroB), purified and crystallized in order to elucidate its three-dimensional structure.

Crystallization and preliminary X-ray crystallographic studies of the ρ-class gluta­thione S-transferase from the Antarctic clam Laternula elliptica

Monoclinic crystals of the ρ-class glutathione S-transferase from L. elliptica were obtained in both glutathione- and CDNB-complexed forms.

Crystallization and preliminary crystallographic studies of the recombinant L-N-carbamoylase from Geobacillus stearothermophilus CECT43

The cloning, expression, purification, crystallization and preliminary X-ray characterization of an L-N-carbamoylase from G. stearothermophilus are described.

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of isocitrate de­hydrogenase 2 (Rv0066c) from Mycobacterium tuberculosis

Isocitrate dehydrogenase 2 from M. tuberculosis was cloned, expressed, purified and crystallized. A complete data set has been collected to 3.25 Å resolution.

Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of malonyl-CoA–acyl carrier protein transacylase (FabD) from Xanthomonas oryzae pv. oryzae

Bacterial blight is the most destructive bacterial disease of rice and is caused by X. oryzae pv. oryzae (Xoo). Xoo0880 (FabD), a malonyl-CoA–acyl carrier protein transacylase, from Xoo was cloned, purified and crystallized.

Crystallization and preliminary X-ray diffraction studies of the prototypal homologue of mitoNEET (Tth-NEET0026) from the extreme thermophile Thermus thermophilus HB8

A thermophilic bacterial homologue of mitoNEET (a mammalian mitochondrial outer membrane protein) from T. thermophilus HB8 (open reading frame TTHA0026; Tth-NEET0026) has been identified as a water-soluble prototypal [2Fe–2S] protein and crystallized. The bipyramidal crystals of the recombinant Tth-NEET0026 diffracted to 1.80 Å resolution using synchrotron radiation.

Cloning, expression, purification, crystallization and preliminary X-ray analysis of NodS N-methyltransferase from Bradyrhizobium japonicum WM9

The NodS N-methyltransferase, an enzyme participating in the biosynthesis of the bacterial nodulation (Nod) factor necessary to establish symbiotic nitrogen fixation with a legume plant host, has been crystallized in the apo form as well as in complex with SAH. SAH is a byproduct of SAM degradation during the SAM-dependent methylation reaction.

Cloning, purification, crystallization and preliminary X-ray analysis of a bacterial GABA receptor with a Venus flytrap fold

A 1.35 Å resolution data set was collected from a crystal of the periplasmic GABA receptor Atu2422 from A. tumefaciens. Atu2422 adopts a closed Venus flytrap conformation.

Crystallization and preliminary X-ray diffraction analysis of a breast cancer metastasis suppressor 1 predicted coiled-coil region

Crystals of human breast cancer metastasis Suppressor 1 N-terminal coiled-coil have been obtained and diffracted to 2.0 Å.

Crystallization and preliminary X-ray analysis of the human androgen receptor ligand-binding domain with a coactivator-like peptide and selective androgen receptor modulators

The human androgen receptor ligand-binding domain has been crystallized as a ternary complex with a coactivator-like undecapeptide and two different synthetic ligands.

Purification, crystallization and preliminary crystallographic analysis of WsaF, an essential rhamnosyltransferase from Geobacillus stearothermophilus

The transfer of sugars is an important process in both biology and biotechnology; the first crystallization of a rhamnosyltransferase is reported.

Crystallization and preliminary X-ray crystallographic analysis of the probable tRNA-modification GTPase (TrmE) from Staphylococcus aureus

In this study, the TrmE protein from the pathogenic bacterium S. aureus was overexpressed and crystallized (space group I23, unit-cell parameters a = b = c = 229.47 Å, α = β = γ = 90°). Diffraction data were collected to 2.9 Å resolution using synchrotron radiation.

Crystallization and preliminary X-ray analysis of endoglucanase from Pyrococcus horikoshii

Hyperthermostable P. horikoshii endoglucanase was produced as a truncated form that maintained enzymatic properties; it was crystallized and its crystals diffracted to 1.78 Å resolution.

Crystallization of the hydantoin transporter Mhp1 from Microbacterium liquefaciens

Mhp1, a hydantoin transporter from M. liquefaciens, was purified and crystallized. Diffraction data were collected to 2.85 Å resolution; the crystal belonged to the orthorhombic space group P2₁2₁2₁.

Crystallization of a ZRANB2–RNA complex

The second RanBP2-type zinc finger from ZRANB2 has been crystallized in complex with a single-stranded RNA target sequence.

Purification, crystallization and preliminary X-ray diffraction analysis of an oomycete-derived Nep1-like protein

The first crystallographic study of a member of the NLP (Nep1-like protein) toxin and elicitor protein family is reported.

Crystallization and preliminary crystallographic study of the phosphoglucose isomerase from Bacillus subtilis

Phosphoglucose isomerase from B. subtilis has been purified and crystallized. The diffraction quality of the crystal was improved by using a flash-annealing technique and 1.9 Å resolution in-house X-ray data were collected.

Purification, crystallization and preliminary X-ray diffraction analysis of the HMG domain of Sox17 in complex with DNA

Crystals of the Sox17 HMG domain bound to LAMA1 enhancer DNA-element crystals that diffracted to 2.75 Å resolution were obtained.