The 1.9 Å structure of the branched-chain amino-acid transaminase (IlvE) from Mycobacterium tuberculosis

The structure of the branched-chain amino-acid transaminase (IlvE) from M. tuberculosis has been resolved at 1.9 Å resolution. The structure, together with sequence conservation, identifies residues that are likely to be responsible for substrate specificity with mechanistic and potential therapeutic relevance.

Structure of the adenylation domain of NAD⁺-dependent DNA ligase from Staphylococcus aureus

The crystal structure of the adenylation domain of NAD⁺-dependent DNA ligase from Staphylococcus aureus reveals a narrow `C2 tunnel' for potential use in inhibitor design.

Structure of an N-terminally truncated Nudix hydrolase DR2204 from Deinococcus radiodurans

The structure of N-terminally truncated DR2204, a Nudix hydrolase, is presented at 2.0–Å resolution.

Structure of an Escherichia coli N-acetyl-D-neuraminic acid lyase mutant, E192N, in complex with pyruvate at 1.45 Å resolution

The 1.45 Å structure of E. coli N-acetyl-D-neuraminic acid lyase in complex with pyruvate in space group P2₁2₁2₁ is reported from new low-salt crystallization conditions that will facilitate soaking experiments with substrates and inhibitors.

Structures of BIR domains from human NAIP and cIAP2

The crystal structures of the human NAIP BIR2 and cIAP2 BIR3 domains have been determined. Both BIR domains harbors an amino-terminal tetrapeptide in its peptide-binding groove.

X-ray structure and biophysical properties of rabbit fibroblast growth factor 1

This report shows that the tertiary structure of rabbit FGF-1 is essentially identical to that of human FGF-1, with four surface mutations (140-amino-acid form). Biophysical data indicate that rabbit FGF-1 is less thermostable than human FGF-1 and has a slower association rate with the FGF-1 receptor. Mitogenic assays indicate that rabbit FGF-1 is tenfold less potent than human FGF-1; however, rabbit FGF-1 exhibits greater heparin stimulation such that its mitogenic activity in the presence of heparin is essentially indistinguishable from that of human FGF-1.

Crystal optimization and preliminary diffraction data analysis of the Smad1 MH1 domain bound to a palindromic SBE DNA element

Crystals of palindromic SBE DNA-bound Smad1 MH1 domain diffracting to 2.7 Å resolution have been obtained.

A preliminary X-ray study of sedoheptulose-7-phosphate isomerase from Burkholderia pseudomallei

Sedoheptulose-7-phosphate isomerase (GmhA) from B. pseudomallei is one of the targets of antibiotic adjuvants for melioidosis. In this study, GmhA has been cloned, expressed, purified and crystallized.

Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of an atypical two-cysteine peroxiredoxin (SAOUHSC_01822) from Staphylococcus aureus NCTC 8325

Diffraction-quality crystals of an atypical two-cysteine peroxiredoxin (SAOUHSC_01822) from S. aureus NCTC 8325 have been obtained. The cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of the protein are reported.

Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major

Overexpression, purification, crystallization and preliminary X-ray diffraction analysis of the nucleoside diphosphate kinase b from Leishmania major are reported. The crystals belonged to the trigonal space group P3₂21 and diffracted to 2.18 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of free me­thionine-(R)-sulfoxide reductase from Staphylococcus aureus

A hexagonal crystal of free methionine-(R)-sulfoxide reductase from S. aureus has been obtained using ammonium sulfate as a precipitant.

Crystallization and preliminary crystallographic analysis of the global nitrogen regulator AmtR from Corynebacterium glutamicum

AmtR is a rare example of a member of the TetR family of bacterial transcription regulators that is not regulated by a small-molecule effector but by interaction with a protein named GlnK. Wild-type and SeMet-substituted AmtR have been produced and crystallized and preliminary electron-density maps have been obtained to 3.0 Å resolution.

Crystallization and preliminary X-ray analysis of the stress-response PPM phosphatase RsbX from Bacillus subtilis

The bacterial PPM phosphatase RsbX from B. subtilis was expressed in E. coli, purified and crystallized. The crystal belonged to space group P1 and diffracted to 1.06 Å resolution.

Recombinant production, crystallization and preliminary X-ray analysis of PCNA from the psychrophilic archaeon Methanococcoides burtonii DSM 6242

The proliferating cell nuclear antigen (PCNA) from the eurypsychrophilic archaeon M. burtonii DSM 6242 has been cloned, overproduced, purified and crystallized. Crystals were deemed to be suitable for X-ray analysis and structure determination to 2.40 Å resolution.

Engineering an improved crystal contact across a solvent-mediated interface of human fibroblast growth factor 1

A solvent-mediated crystal contact in fibroblast growth factor-1 was subjected to mutagenesis to improve crystal growth. The results indicate that improved growth was achieved upon elimination of the solvent-mediated interface and introduction of direct crystal contacts.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of D-ribose-5-phosphate isomerase from Clostridium thermocellum

Clostridium thermocellum D-­ribose-5-phosphate isomerase has been purified and crystallized in order to determine its three-dimensional structure and thus to elucidate its enzymatic reaction mechanism and understand its substrate specificity.

Expression, purification, crystallization and preliminary X-ray analysis of conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 0708

Conjugated polyketone reductase C2 from C. parapsilosis IFO 0708 was expressed, purified and crystallized by the sitting-drop vapour-diffusion method. The crystal belonged to space group P2₁2₁2₁ and diffracted X-rays to 1.7 Å resolution.

Crystallization of the head and galectin-like domains of porcine adenovirus isolate NADC-1 fibre

The head and predicted galectin-like domains of porcine adenovirus NADC-1 isolate fibre have been independently crystallized. Diffraction data have been obtained to 3.2 Å resolution for the head-domain crystals and to 1.9 Å resolution for the galectin-like domain crystals.

Purification, crystallization and preliminary X-ray analysis of bifunctional isocitrate de­hydrogenase kinase/phosphatase in complex with its substrate, isocitrate de­hydrogenase, from Escherichia coli

The protein complex of bifunctional isocitrate dehydrogenase kinase/phosphatase with its substrate, isocitrate dehydrogenase, has been crystallized for structural analysis. A complete data set was collected from the complex crystal and processed to 2.9 Å resolution.

Crystallization and preliminary X-ray crystallographic characterization of a public CMV-specific TCR in complex with its cognate antigen

In order to validate the hypothesis of a shared recognition mode for the CMV immunodominant NLV-HLA-A2 epitope by specific high-avidity T-cell clones, a second public TCR, RA15, in complex with NLV-HLA-A2 was overexpressed, purified and crystallized. Preliminary diffraction data obtained at 4.7 Å resolution and a molecular-replacement solution suggest an overall similar docking mode for RA15 TCR on NLV-HLA-A2 compared with the public RA14 TCR, for which the structure has previously been solved.

Crystallization and preliminary X-ray diffraction analysis of the fructo­furanosidase from Schwanniomyces occidentalis

The invertase from Schwanniomyces occidentalis has been expressed in Saccharomyces cerevisiae, purified and crystallized. The wild-type enzyme was also purified and crystallized and diffraction data were collected to 2.9 Å resolution.

Purification, crystallization and preliminary X-ray diffraction analysis of disease-related mutants of p97

Mutations in the human AAA+ protein p97 cause a disease in humans called IBMPFD. How these mutations affect the structure and function of p97 is unknown. Here, the crystallization of three disease-related mutants of p97 in the presence of ATPγS are reported.

Crystallization and preliminary crystallographic analysis of gallate dioxygenase DesB from Sphingobium sp. SYK-6

Gallate dioxygenase (DesB) from Sphingobium sp. SYK-6, which belongs to the type II extradiol dioxygenase family, was purified and crystallized in two different crystal forms, which were subjected to X-ray analysis.

Crystallization and preliminary crystallographic studies of di­hydrofolate reductase-thymidylate synthase from Trypanosoma cruzi, the Chagas disease pathogen

Crystals of complexes of the T. cruzi dihydrofolate reductase-thymidylate synthase enzyme with three antifolates in two space groups have been obtained that diffracted to 2.1–2.8 Å resolution. The antifolates used for cocrystallization were dihydrotriazine-based and quinazoline-based antifolates.

Crystallization and preliminary X-ray diffraction analysis of the complex of Kunitz-type tamarind trypsin inhibitor and porcine pancreatic trypsin

A complex of tamarind trypsin inhibitor with porcine trypsin was crystallized and analyzed by X-ray diffraction.

Purification, crystallization and preliminary X-ray diffraction experiments on the breakage-reunion domain of the DNA gyrase from Mycobacterium tuberculosis

The breakage-reunion domain of M. tuberculosis DNA gyrase was crystallized using the hanging-drop vapour-diffusion method. One of the four crystal forms obtained belonged to space group C2 and diffraction data were collected to a resolution of 2.7 Å.

Crystallization and initial X-ray diffraction study of the three PASTA domains of the Ser/Thr kinase Stk1 from the human pathogen Staphylococcus aureus

Crystallization conditions have been determined for an extracellular portion of the Ser/Thr kinase Stk1 from the human pathogen S. aureus that contains three PASTA subunits. Synchrotron data have been collected to a resolution of 2.9 Å. Phasing is in progress.

Purification, crystallization and preliminary X-ray analysis of β-glucosidase from Kluyveromyces marxianus NBRC1777

The preliminary X-ray analysis of β-glucosidase (BglI) from K. marxianus, which belongs to glycoside hydrolase family 3, is described.

Expression, purification and crystallization of an archaeal-type phosphoenolpyruvate carboxylase

The expression, purification, crystallization and preliminary diffraction analysis of an archaeal-type phosphoenolpyruvate carboxylase are described. Complete highly redundant X-ray data have been measured from a crystal diffracting to 3.13 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of γ-carboxymucolactone de­carboxylase from Sulfolobus solfataricus

γ-Carboxymucolactone decarboxylase from the thermophilic archaeon S. solfataricus was crystallized. Diffraction to 2.40 Å resolution was obtained from a flash-cooled crystal using synchrotron radiation.