Structure of photosynthetic glyceraldehyde-3-phosphate de­hydrogenase (isoform A₄) from Arabidopsis thaliana in complex with NAD

The crystal structure of the photosynthetic A₄ isoform of glyceraldehyde-3-phosphate dehydrogenase from the model plant A. thaliana has been solved at 2.6 Å resolution. The tetrameric structure includes four molecules of NAD and eight sulfate ions occupying the P sites involved in catalysis.

The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin

A high-resolution structure of P. pantrotrophus pseudoazurin is reported at 1.4 Å resolution, together with secondary-structure-based sequence alignment of the type 1 copper protein family.

Structure of Stenotrophomonas maltophilia FeoA complexed with zinc: a unique prokaryotic SH3-domain protein that possibly acts as a bacterial ferrous iron-transport activating factor

The crystal structure of FeoA from Stenotrophomonas maltophilia has been determined to a resolution of 1.7 Å using an Se single-wavelength anomalous dispersion (Se-SAD) approach and revealed a unique dimer cross-linked by two zinc ions and six chloride ions.

The 1.35 Å resolution structure of the phosphatase domain of the suppressor of T-cell receptor signaling protein in complex with sulfate

The crystal structure of the phosphatase domain of Sts-1 at pH 4.6 complexed with sulfate has been determined to 1.35 Å resolution.

The structure of PhaZ7 at atomic (1.2 Å) resolution reveals details of the active site and suggests a substrate-binding mode

The crystal structure of PhaZ7 depolymerase determined at atomic (1.2 Å) resolution in the presence of PMSF reveals a preformed serine protease catalytic triad and details of the architecture of the active site.

Expression, purification and crystallization of a thermostable short-chain alcohol de­hydrogenase from the archaeon Thermococcus sibiricus

The expression, purification and crystallization of a thermostable short-chain alcohol dehydrogenase from the archaeon T. sibiricus is reported.

Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of Mrs2, a magnesium ion transporter from yeast inner mitochondrial membrane

Soluble N-terminal domain of yeast magnesium ion transporter Mrs2 was overexpressed in E. coli, purified, crystallized and X-ray diffraction data collected to 1.83 Å resolution.

Crystallization and preliminary crystallographic studies of the butyrolactone autoregulator receptor protein (BarA) from Streptomyces virginiae

A DNA-binding protein from S. virginiae was crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to approximately 3.0 Å resolution.

Incorporation of methyl-protonated valine and leucine residues into deuterated ocean pout type III antifreeze protein: expression, crystallization and preliminary neutron diffraction studies

Deuterated type III antifreeze protein specifically hydrogen reverse-labelled in the methyl groups of leucine and valine residues has been expressed, purified and crystallized. Preliminary neutron data collection showed diffraction to 1.80 Å resolution from a 0.23 mm³ crystal.

Crystallization and preliminary crystallographic analysis of the catalytic module of endolysin from Cp-7, a phage infecting Streptococcus pneumoniae

The catalytic module of endolysin from the phage Cp-7 was crystallized using the vapour-diffusion method. Native data were collected to 2.4 Å resolution.

Expression, crystallization and preliminary crystallographic study of mouse hepatitis virus (MHV) nucleocapsid protein C-terminal domain

The C-terminal domain of mouse hepatitis virus nucleocapsid protein has been overexpressed in E. coli, purified and crystallized. The crystal belonged to space group P422, with unit-cell parameters a = 66.6, c = 50.8 Å, and diffracted to 2.20 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of L-rhamnose isomerase with a novel high thermostability from Bacillus halodurans

L-Rhamnose isomerase (L-RhI) from B. halodurans has been purified and crystallized. The crystals of L-RhI belonged to the monoclinic space group P2₁, with unit-cell parameters a = 83.2, b = 164.9, c = 92.0 Å, β = 116.0°, and diffracted to 2.5 Å resolution.

Crystallization and preliminary crystallographic studies of CorC, a magnesium-ion transporter

Different forms of the magnesium-ion transporter CorC have been crystallized; crystallization was affected by nucleotides and magnesium ions.

Expression, purification, crystallization and preliminary X-ray analysis of the EIIC^Glc domain of the Escherichia coli glucose transporter

The transporters of the bacterial phosphotransferase system mediate the uptake of carbohydrates with concomitant phosphorylation. Good-quality crystals have been obtained of the membrane-spanning EIIC^Glc domain of the E. coli glucose transporter.

Crystallization and preliminary X-ray analysis of Ebola VP35 interferon inhibitory domain mutant proteins

Three mutant forms of Ebola VP35 interferon inhibitory domain were crystallized in three different space groups.

Crystallization of a 45 kDa peroxygenase/peroxidase from the mushroom Agrocybe aegerita and structure determination by SAD utilizing only the haem iron

The glycosylated fungal haem-thiolate peroxygenase AaPII was crystallized in three different forms from a charge-heterogeneous protein sample. Structure solution of the 45 kDa protein was achieved with SAD utilizing the haem iron and the structure was determined at 2.2 Å resolution.

Crystallization and preliminary X-ray crystallographic studies of a Lys49-phospholipase A₂ homologue from Bothrops pirajai venom complexed with rosmarinic acid

PrTX-I, a noncatalytic and myotoxic Lys49-phospholipase A₂ from B. pirajai venom, was cocrystallized with the inhibitor rosmarinic acid from C. verbenacea. The crystals diffracted X-rays to 1.8 Å resolution and the structure was solved, indicating a remarkable electronic density for the ligand at the entrance to the hydrophobic channel.

Expression, purification and crystallization of human prolylcarboxypeptidase

Crystallization of glycosylated human prolylcarboxypeptidase expressed in Chinese hamster ovary cells is described. The hexagonal crystals belong to space group R32 and diffract to 2.8 Å resolution.

Crystallization and preliminary X-ray diffraction studies of infectious bronchitis virus nonstructural protein 9

The gene segment encoding avian infectious bronchitis virus nonstructural protein 9 has been cloned and expressed in Escherichia coli. The protein has been crystallized and the crystals diffracted X-rays to 2.44 Å resolution.

Expression, crystallization and preliminary X-ray crystallographic study of ethanolamine ammonia-lyase from Escherichia coli

Ethanolamine ammonia-lyase from E. coli has been overexpressed, purified and crystallized. The crystals diffracted to 2.2 Å resolution using synchrotron radiation.

Crystallization and preliminary X-ray diffraction studies of a ferredoxin reductase component of carbazole 1,9a-dioxygenase from Novosphingobium sp. KA1

The ferredoxin reductase component of carbazole 1,9a-dioxygenase (Red) is involved in electron transfer from NAD(P)H to ferredoxin. The class IIA Red from Novosphingobium sp. KA1 was crystallized and the crystal diffracted to a resolution of 1.58 Å.

Crystallization and preliminary X-ray crystallographic analysis of highly thermostable L2 lipase from the newly isolated Bacillus sp. L2

Thermostable recombinant L2 lipase from thermophilic Bacillus sp. L2 has been crystallized by using counter-diffusion method and diffracted to 2.7 Å resolution. The crystal belongs to the primitive orthorhombic space group P2₁2₁2₁ with unit-cell parameters a = 87.44, b = 94.90, c = 126.46 Å.

Crystallization and preliminary X-ray analysis of SDR-type pyridoxal de­hydrogenase from Mesorhizobium loti

SeMet-substituted pyridoxal 4-dehydrogenase from M. loti MAFF303099 was crystallized and diffraction data were collected to 2.9 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of a full-length active form of the Cry4Ba toxin from Bacillus thuringiensis

The crystallization of the Cry4Ba toxin from B. thuringiensis is described.

Purification, crystallization and preliminary X-ray analysis of human GIMAP2

GIMAP2, a guanine nucleotide-binding protein belonging to the GIMAP family, has been crystallized in various nucleotide-loaded states.

Crystallization and preliminary X-ray diffraction analysis of the complex of a human anti-ephrin type-A receptor 2 antibody fragment and its cognate antigen

Crystals of the complex between the Fab fragment of a human anti-EphA2 antibody and the N-terminal domain of human EphA2 have been obtained. Diffraction data were collected to 2.55 Å resolution.

Expression, purification, crystallization and preliminary X-ray analysis of ORF60, the small subunit (R2) of ribonucleotide reductase from Kaposi's sarcoma-associated herpesvirus (KSHV)

Crystals of the R2 subunit from the oncovirus Kaposi's sarcoma-associated γ-herpesvirus (KSHV) were obtained by the use of in situ proteolysis. The crystals diffracted to 2.0 Å resolution and belonged to space group P2₁.

Crystallization and preliminary X-ray diffraction analysis of various enzyme–substrate complexes of iso­propylmalate de­hydrogenase from Thermus thermophilus

The enzyme 3-isopropylmalate dehydrogenase (IPMDH) from T. thermophilus, which catalyses the penultimate reaction step of the leucine-biosynthesis pathway, has been crystallized in various states along its reaction coordinate.

Comparative analysis of amino acids and amino-acid derivatives in protein crystallization

New types of aggregation suppressors, such as amino acids and their derivatives, were focused on as fourth-component additives. Data were obtained that indicated that the additives promote protein crystallization.

Amino acids and glycine ethyl ester as new crystallization reagents for lysozyme

During the past two decades, amino acids and amino-acid derivatives have been applied in various fields of protein chemistry. The potential use of amino acids and their derivatives as new precipitating agents is described.