Structures of engineered Clostridium botulinum neurotoxin derivatives

The crystal structures of engineered C. botulinum neurotoxin–SNARE derivatives have been and exhibit strong stability of the LHn fragment.

Structure of hyperthermophilic β-glucosidase from Pyrococcus furiosus

Recombinant hyperthermophilic β-glucosidase from P. furiosus was crystallized. The crystal structure was solved to a resolution of 2.35 Å.

The 1.7 Å resolution structure of At2g44920, a pentapeptide-repeat protein in the thylakoid lumen of Arabidopsis thaliana

The crystal structure of At2g44920, a pentapeptide repeat protein (PRP) from Arabidopsis thaliana, has been determined at 1.7 Å resolution. The structure represents the first PRP protein whose subcellular localization has been experimentally confirmed to be the thylakoid lumen of a plant species.

Engineered tryptophan in the adenine-binding pocket of catalytic subunit A of A-ATP synthase demonstrates the importance of aromatic residues in adenine binding, forming a tool for steady-state and time-resolved fluorescence spectroscopy

The crystallographic structures of the subunit B mutants F427W and F508W of the Pyrococcus horikoshii OT3 of the A1AO ATP synthase reveal that the exact volume of the adenine ribose binding pocket is essential for ATP-/ADP-binding.

Therapeutic target-site variability in α₁-antitrypsin characterized at high resolution

A new 1.8 Å resolution structure of α₁-antitrypsin demonstrates structural variability within an allosteric site in the molecule.

A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase

Structural analysis of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15.

The structure of LsrB from Yersinia pestis complexed with autoinducer-2

The structure of LsrB from Y. pestis complexed with autoinducer-2 (AI-2) has been determined at 1.75 Å resolution. Bound AI-2 adopts the (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran conformation, which is the same conformation as is observed in the S. typhimurium and S. meliloti LsrB–AI-2 structures.

Structure of d(CGGGTACCCG)₄ as a four-way Holliday junction

The crystal structure of the DNA sequence d(CGGGTACCCG)₄ as a four-way Holliday junction and its interaction with a calcium ion are described.

The structure of an N11A mutant of the G-protein domain of FeoB

The structure of NFeoB from S. thermophilus harbouring an N11A mutation has been determined to 1.85 Å resolution.

Crystallization and preliminary X-ray structure analysis of human ribosomal protein L30e

The purification, crystallization and X-ray structure analysis of human L30e are presented here.

Structure of the H107R variant of the extracellular domain of mouse NKR-P1A at 2.3 Å resolution

The crystal structure of the H107R variant of the extracellular domain of mouse NKR-P1A was determined using X-ray diffraction from a merohedrally twinned crystal.

Structure of the fucose mutarotase from Streptococcus pneumoniae in complex with L-fucose

SpFcsU is a fucose mutarotase from S. pneumoniae that links the harvesting of fucose from glycans by glycoside hydrolases to processing of the fucose monosaccharide by subsequent enzymes in a fucose-utilization pathway. Here, the decameric structure of SpFcsU in complex with fucose is described.

Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of the periplasmic domain of outer membrane protein A from Acinetobacter baumannii

The crystallization of the OmpA periplasmic domain from A. baumannii is described.

Purification, crystallization and preliminary X-ray characterization of a haemagglutinin from the seeds of Jatropha curcas

A novel haemagglutinin from Jatropha curcas seeds is purified and crystallized. X-ray diffraction data collected from the rod-shaped crystals were processed in the orthorhombic space group P2₁2₁2₁ and the crystals diffracted to 2.8 Å resolution at 103 K.

Cloning, expression, purification and crystallization of di­hydrodipicolinate synthase from the grapevine Vitis vinifera

Dihydrodipicolinate synthase from the common grapevine V. vinifera has been cloned, expressed, purified and crystallized in the presence of the substrate pyruvate by in-drop hexahistidine-tag cleavage. A diffraction data set has been collected to a resolution of 2.2 Å.

Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans

S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2₁.

Crystallization and preliminary X-ray analysis of crinumin, a chymotrypsin-like glycosylated serine protease with thrombolytic and antiplatelet activity

The crystallization and preliminary X-ray diffraction analysis of crinumin, a plant serine protease, is reported.

Crystallization and preliminary X-ray crystallographic analysis of β-keto­acyl-ACP synthase I (XoFabB) from Xanthomonas oryzae pv. oryzae

β-Ketoacyl-acyl carrier protein synthase I (XoFabB) from X. oryzae pv. oryzae (Xoo) plays a crucial role in fatty-acid synthesis and has been considered as a target for the development of antibacterial agents against Xoo. XoFabB was expressed, purified and crystallized to determine its atomic resolution structure.

Overexpression, purification, crystallization and preliminary crystallographic studies of a hyperthermophilic adenylosuccinate synthetase from Pyrococcus horikoshii OT3

A hyperthermophilic adenylosuccinate synthetase from P. horikoshii OT3, which is 90–120 amino acids shorter than those from the vast majority of organisms, was expressed, purified and crystallized and X-ray diffraction data were collected to 2.5 Å resolution.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of shikimate de­hydrogenase from Archaeoglobus fulgidus

Shikimate dehydrogenase from A. fulgidus has been overexpressed and crystallized. X-ray diffraction data have been collected to 2.8 Å resolution.

Crystallization and preliminary crystallographic analysis of a putative glucokinase/hexokinase from Thermus thermophilus

In this study, a putative glucokinase/hexokinase from T. thermophilus was purified and crystallized. Diffraction data were collected and processed to 2.02 Å resolution.

Crystallization and preliminary X-ray crystallographic studies of VibE, a vibriobactin-specific 2,3-di­hydroxybenzoate-AMP ligase from Vibrio cholerae

This article reports the molecular cloning, protein expression and purification, crystallization and preliminary X-ray crystallographic analysis of the vibriobactin synthetase VibE from V. cholerae.

Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-TΨC domain of tRNA

Peptidyl-tRNA hydrolase from E. coli has been crystallized in complex with the acceptor-TΨC domain of tRNA. Diffraction data have been collected and processed to 2.4 Å resolution.

Improved X-ray diffraction from Bacillus megaterium penicillin G acylase crystals through long cryosoaking dehydration

Penicillin G acylase from the Gram-positive bacterium B. megaterium was crystallized and X-ray diffraction from these crystals could be substantially improved by slight dehydration through a long cryo-soak.

Crystallization and preliminary crystallographic analysis of a C2 protein from Arabidopsis thaliana

A C2 protein from A. thaliana has been crystallized by the hanging-drop vapour-diffusion method and a native data set has been collected at 2.4 Å resolution.

Crystallization and preliminary crystallographic studies of the NAD⁺-dependent de­acetylase HST1 from Saccharomyces cerevisiae

The Saccharomyces cerevisiae NAD⁺-dependent deacetylase HST1_156–503 was expressed and crystallized. Crystals grown by the hanging-drop vapour-diffusion method diffracted to 2.90 Å resolution.

Expression, purification, crystallization and preliminary X-ray diffraction studies of the human keratin 4-binding domain of serine-rich repeat protein 1 from Streptococcus agalactiae

Expression, purification and crystallization of Srr-1-K4BD, a human keratin 4-binding domain of serine-rich repeat protein 1 from S. agalactiae, was carried out. Native crystals of Srr-1-K4BD diffracted to 3.8 Å resolution using synchrotron radiation.

Crystallization and preliminary X-ray analysis of crustacean hyperglycaemic hormone from the kuruma prawn Marsupenaeus japonicus in its weakly active precursor form

Crustacean hyperglycaemic hormone from the kuruma prawn M. japonicus was crystallized by the sitting-drop vapour-diffusion method in its weakly active precursor form which has an extra glycine residue at the C-terminus. The crystals belonged to the orthorhombic space group P2₁2₁2₁ and diffracted to 1.95 Å resolution.

Expression, purification, crystallization and preliminary X-ray diffraction crystallographic study of PurH from Escherichia coli

Tthe cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of a bifunctional purine-biosynthesis enzyme from E. coli which possesses aminoimidazole-4-carboxamide ribonucleotide transformylase and IMP cyclohydrolase activities are reported.

Thermostable multicopper oxidase from Thermus thermophilus HB27: crystallization and preliminary X-ray diffraction analysis of apo and holo forms

A thermostable multicopper oxidase from Thermus thermophilus HB27 (Tth-MCO) has been successfully crystallized using the sitting-drop and hanging-drop vapour-diffusion methods.

Crystallization of Chlorella deoxyuridine triphosphatase

The crystallization of Chlorella dUTPase is described. The crystallization of plant dUTPase isozymes and their parameterization is discussed.

Crystallization and X-ray diffraction analysis of the C-terminal domain of the flax rust effector protein AvrM

This report describes the crystallization and X-ray diffraction analysis of two different allelic variants of the AvrM effector from flax rust.

Crystallization and preliminary X-ray diffraction analysis of Val57 mutants of the amyloidogenic protein human cystatin C

The crystallization and preliminary X-ray diffraction analysis of Val57 mutants of human cystatin C, designed to assess the influence of changes in the properties of loop L1 on dimerization propensity, are reported.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a major group 7 allergen, Der f 7, from the dust mite Dermatophagoides farinae

The major group 7 allergen, Der f 7, from the dust mite Dermatophagoides farinae has been crystallized and diffracted X-rays to a resolution of 2.24 Å.

Purification, crystallization and preliminary X-ray diffraction studies of the arsenic repressor ArsR from Corynebacterium glutamicum

The cysteine free derivative of the arsenic repressor ArsR from Corynebacterium glutamicum was expressed, purified, crystallized and X-ray diffraction data up to 1.86 Å resolution have been collected. The crystals belonged to the space group P4 with the unit-cell parameters a = b = 41.84, c = 99.47 Å.

Crystallization and X-ray data collection of HP0902 from Helicobacter pylori 26695

Two types of His₆-tag-fused HP0902 protein from H. pylori 26695 have been crystallized and X-ray diffraction data were obtained at 1.4 and 2.5 Å resolution.

Cloning, purification and crystallographic analysis of a hypothetical protein, BPSL1549, from Burkholderia pseudomallei

B. pseudomallei BPSL1549 has been overexpressed in E. coli, purified and crystallized.

Purification, crystallization and preliminary X-ray diffraction of fluorescence recovery protein from Synechocystis PCC 6803

Fluorescence recovery protein from Synechocystis PCC 6803 plays a key role in the orange carotenoid protein-related photoprotective mechanism in cyanobacteria. The full-length form and a truncated form were overexpressed, purified and crystallized, and diffraction was observed to 2.75 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of mouse prostaglandin F_2α synthase, AKR1B3

Aldo-keto reductase 1B3 (AKR1B3) produced in Escherichia coli has been crystallized in complex with NADPH by the sitting-drop vapour-diffusion method.

Expression, crystallization and preliminary crystallographic analysis of C-reactive protein from zebrafish

Crystals of native and selenomethionine-substituted C-reactive protein from zebrafish diffracted to 2.3 and 1.7 Å resolution, respectively, and belonged to space group R3 with one molecule per asymmetric unit. The Matthews coefficient was calculated to be 3.28 ų Da⁻¹.

Expression, purification and preliminary crystallographic analysis of Rv2247, the β subunit of acyl-CoA carboxylase (ACCD6) from Mycobacterium tuberculosis

The acyl-CoA carboxylase β subunit (ACCD6) of M. tuberculosis has been crystallized and preliminary X-ray crystallographic analysis has been performed.

Purification, crystallization and preliminary crystallographic analysis of peroxidase from the palm tree Chamaerops excelsa

Diffraction-quality crystals of the peroxidase from the palm tree C. excelsa were obtained and a native X-ray diffraction data set was collected at a synchrotron source.

Overexpression, purification, crystallization and preliminary X-ray analysis of CheY4 from Vibrio cholerae O395

The chemotaxis response regulator CheY4 from V. cholerae has been cloned, overexpressed, purified and crystallized in monoclinic and hexagonal space groups; the crystals diffracted to 1.67 and 1.9 Å resolution, respectively.

Crystallization and preliminary X-ray crystallographic analysis of a helicase-like domain from a tomato mosaic virus replication protein

Crystals of the helicase domain from a tomato mosaic virus replication protein obtained using the hanging-drop vapour-diffusion method at 285 K diffracted X-rays to 2.05 Å resolution. They belonged to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 85.8, b = 128.3, c = 40.7 Å.

Crystallization of a 79 kDa fragment of the hook protein FlgE from Campylobacter jejuni

A 79 kDa fragment of FlgE from C. jejuni has been crystallized.

Preliminary X-ray crystallographic studies on acetate kinase (AckA) from Salmonella typhimurium in two crystal forms

Acetate kinase from S. typhimurium was crystallized in two crystal forms that diffracted to 2.70 Å (form I) and 1.90 Å (form II) resolution.

In-house SAD phasing with surface-bound cerium ions

Cerium was used to enhance the anomalous signal in hen egg-white lysozyme crystals and led to successful in-house SAD phasing.

Seleno-detergent MAD phasing of leukotriene C₄ synthase in complex with do­decyl-β-D-selenomaltoside

Dodecyl-β-D-selenomaltoside in a leukotriene C₄ synthase crystal exhibited sufficient anomalous diffraction for multiwavelength anomalous diffraction phasing.

Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate de­hydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate. Corrigendum

A correction to the article by Ortíz et al. [(2011), Acta Cryst. F67, 1457–1461].