Structure of human tankyrase 1 in complex with small-molecule inhibitors PJ34 and XAV939

Crystal structures of human tankyrase I in complex with small-molecule inhibitors PJ34 and XAV939 have been determined, both to 2.0 Å. The TNKS1-PJ34 system allows for displacement soaking and, therefore, is an ideal system for structure-based drug design.

Room-temperature ultrahigh-resolution time-of-flight neutron and X-ray diffraction studies of H/D-exchanged crambin

The 0.85 Å room-temperature ultrahigh-resolution structure of H/D-exchanged crambin is reported. Preliminary 1.1 Å resolution neutron diffraction data have been collected at the neutron Protein Crystallography Station at LANSCE.

Structures of new crystal forms of Mycobacterium tuberculosis peptidyl-tRNA hydrolase and functionally important plasticity of the molecule

The structures of new crystal forms of M. tuberculosis peptidyl-tRNA hydrolase confirm and provide further elaboration of the functionally relevant plasticity of the enzyme molecule.

Structure of a pectin methylesterase from Yersinia enterocolitica

The first crystal structure of a pectin methylesterase from an enteropathogen is presented. This enzyme from Y. enterocolitica has biological significance for the evolution of pectin-metabolic pathways within pectinolytic bacteria and related agents of foodborne illness.

A new crystal lattice structure of Helicobacter pylori neutrophil-activating protein (HP-NAP)

A new crystal lattice structure of H. pylori neutrophil-activating protein has been determined. Iron loading causes a series of conformational changes at the ferroxidase centre.

Three-dimensional structure of a thermophilic family GH11 xylanase from Thermobifida fusca

The structure of a thermostable xylanase from T. fusca is reported.

Structure of the interleukin-2 tyrosine kinase Src homology 2 domain; comparison between X-ray and NMR-derived structures

The interleukin-2 tyrosine kinase Src homology 2 domain was crystallized and its structure was solved to 2.35 Å resolution. The structure reveals a domain-swapped dimer that is related to other dimeric SH2 domains solved previously. The cis–trans-prolyl isomerization that is evident from solution studies of Itk SH2 cannot be observed in the crystal structure.

Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin–Arg–lysozyme complex

An unusual case of trace amounts of a contaminating protein facilitating formation of a heterotrimeric protein complex.

Cloning, purification, crystallization and preliminary X-ray diffraction analysis of mouse PACSIN 3 protein

The N-terminal F-BAR domain of mouse PACSIN 3, which contains 341 amino acids, has been successfully cloned, purified and crystallized.

Expression, purification, crystallization and preliminary crystallographic analysis of a thermostable DNA ligase from the archaeon Thermococcus sibiricus

A recombinant ATP-dependent DNA ligase from the hyperthermophilic anaerobic archaeon Thermococcus sibiricus was expressed in Escherichia coli and purified. Crystals were grown by vapour diffusion using the hanging-drop method.

Crystallization of the C-terminal domain of the bacteriophage T7 fibre protein gp17

The C-terminal domain of the bacteriophage T7 fibre protein gp17, consisting of amino acids 371–553, has been crystallized. Diffraction data have been obtained to around 2.0 Å resolution from two different crystal forms. Multiwavelength anomalous dispersion phasing with a mercury derivative is in progress.

Crystallization and preliminary X-ray diffraction analysis of the metalloregulatory protein DtxR from Thermoplasma acidophilum

Orthorhombic crystals of DtxR from T. acidophilum have been obtained. X-ray data were collected to 1.8 Å resolution using synchrotron radiation.

Crystallization and preliminary crystallographic analysis of LipC12, a true lipase isolated through a metagenomics approach

The preliminary X-ray analysis of LipC12, the first lipase isolated through a metagenomics approach to be crystallized, is reported.

Purification, crystallization and preliminary X-ray crystallographic studies of the Mycobacterium tuberculosis DNA gyrase CTD

The M. tuberculosis DNA gyrase A C-terminal domain (CTD) was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group P2₁2₁2₁ and diffraction data were collected to a resolution of 1.55 Å.

Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa PelD

The expression, purification and crystallization of a soluble construct of the putative inner membrane protein PelD from P. aeruginosa is described. The crystals diffracted to a resolution of 2.2 Å.

Crystallization of oligonucleotides containing A-rich repeats suggests a structural contribution to the autoregulation mechanism of PABP translation

Several DNA and RNA fragments containing tandem AAAAAA repeats, which were synthesized as analogues of the A-rich repeats found in the autoregulatory sequence of PABP mRNA, have been crystallized, suggesting the possibility that the autoregulatory sequence has a specific structure which impedes the binding of PABP.

Crystallization and X-ray diffraction analysis of chondroitin lyase from baculovirus: envelope protein ODV-E66

Baculovirus envelope protein ODV-E66 (67–704) was crystallized; the crystal diffracted to 1.8 Å resolution and belonged to space group P6₂ or P6₄, with unit-cell parameters a = b = 113.5, c = 101.5 Å.

Crystallization and preliminary X-ray crystallographic analysis of dioscorin from Dioscorea japonica

Dioscorin from D. japonica was expressed, purified and crystallized using the sitting-drop vapour-diffusion method. The dioscorin crystal diffracted X-rays to 2.11 Å resolution.

Purification, crystallization and preliminary X-ray characterization of Bacillus cereus arylamine N-acetyltransferase 3 [(BACCR)NAT3]

B. cereus arylamine N-acetyltransferase 3 was expressed, purified and crystallized. X-ray diffraction data were collected to 2.42 Å resolution and the crystals belonged to the monoclinic space group C121.

Crystallization and preliminary X-ray analysis of S-ribosylhomocysteinase from Streptococcus mutans

S-Ribosylhomocysteinase (LuxS) encoded by the LuxS gene from Streptococcus mutans was solubly expressed in Escherichia coli, purified and crystallized. Diffraction by the crystal extended to 2.4 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an L-amino-acid ligase

Native and selenomethionine-derivatized (SeMet) crystals of Bacillus subtilis YwfE in the presence of ADP, MgCl₂ and the dipeptide L-Ala-L-Gln were obtained using the hanging-drop vapour-diffusion method.

Retracted: Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of aspartyl aminopeptidase from the apeB gene of Pseudomonas aeruginosa

Aspartyl aminopeptidase (APP) is a metallopeptidase that catalyzes the hydrolysis of aspartic acid from the N-termini of polypeptide chains. The apeB gene from P. aeruginosa was cloned, the APP enzyme was expressed and crystallized and a preliminary X-ray crystallographic study was performed in order to understand its unique reaction pathway.

Crystallization and preliminary X-ray diffraction analysis of an L-amino-acid oxidase from Bothrops jararacussu venom

An L-amino-acid oxidase from B. jararacussu venom was crystallized and X-ray diffraction data were collected to a maximum resolution of 3.0 Å.

Crystallization and preliminary X-ray diffraction analysis of human dipeptidyl peptidase 10 (DPPY), a component of voltage-gated potassium channels

The expression, purification and crystallization of human dipeptidyl peptidase 10, a component of voltage-gated potassium channels, is described.

Expression, purification and preliminary structural analysis of the coiled-coil domain of Deinococcus radiodurans RecN

The coiled-coil domain of the essential DNA-repair protein RecN from D. radiodurans was crystallized. The crystals belonged to space group P2₁ and diffracted X-rays to 2.04 Å resolution.

Crystallization and X-ray diffraction analysis of a novel surface-adhesin protein: protein E from Haemophilus influenzae

Protein E of the respiratory pathogen H. influenzae is a multifunctional adhesin that is involved in bacterial attachment to host epithelium and direct interactions with vitronectin, laminin and plasminogen. The method of crystallization and X-ray data collection for protein E at 1.8 Å is presented.

Crystallization and preliminary X-ray analysis of the Clostridium botulinum type D nontoxic nonhaemagglutinin

The nontoxic nonhaemagglutinin of serotype D neurotoxin complex, which is important in proteolytic stability for toxin complex, was expressed in E. coli, and crystallized.

Refolding, purification and crystallization of the FrpB outer membrane iron transporter from Neisseria meningitidis

The refolding, purification and crystallization of FrpB from the meningitis pathogen Neisseria meningitidis is described.

Purification, crystallization and preliminary X-ray diffraction analysis of the Hsp40 protein CPIP1 from Nicotiana tabacum

CPIP1 from N. tabacum (NtCPIP1) is a plant heat-shock 40-type protein that participates in effector–host interactions during potyviral infections. Whereas crystals of full-length wild-type NtCPIP1 only diffracted to 8.0 Å resolution, diffraction from crystals of a shortened variant lacking the first 127 amino acids extended to 2.4 Å resolution.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of a ribokinase from the thermohalophile Halothermothrix orenii

A ribokinase gene (rbk) from the anaerobic halothermophilic bacterium Halothermothrix orenii was cloned and overexpressed in Escherichia coli. The recombinant protein (Ho-Rbk) was purified using immobilized metal-ion affinity chromatography and crystals were obtained using the sitting-drop method.

Crystallization and preliminary X-ray diffraction analysis of orotate phosphoribosyltransferase from the human malaria parasite Plasmodium falciparum

Orotate phosphoribosyltransferase from Plasmodium falciparum produced in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method in complex with OA and PRPP in the presence of Mg²⁺.

Notes for authors 2012