Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus

The two recombinant apo subunits H1 and H2 from H. americanus have been structurally characterized. Reconstitution studies with astaxanthin reproduced the bathochromic shift of 85–95 nm typical of the natural crustacyanin subunits.

Structure of Aeropyrum pernix fibrillarin in complex with natively bound S-adenosyl-L-me­thionine at 1.7 Å resolution

The crystal structure of A. pernix fibrillarin includes a tightly bound S-adenosyl-L-methionine molecule.

Flexibility of the P-loop of Pim-1 kinase: observation of a novel conformation induced by interaction with an inhibitor

The structures of Pim1 kinase in complex with in silico screening hits and several subsequently optimized inhibitors, are reported.

Structure of human Rack1 protein at a resolution of 2.45 Å

The crystal structure of human Rack1 protein is reported at 2.45 Å resolution.

Anomalous dispersion analysis of inhibitor flexibility: a case study of the kinase inhibitor H-89

The anomalous dispersion signal of the bromine-containing kinase inhibitor H-89 was used to characterize discrete binding modes of the compound when complexed with the catalytic subunit of protein kinase A.

Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K

The first structure of a fluorescent protein derived from A. victoria green fluorescent protein bearing the obligate-monomer mutation A206K is presented and shows no significant structural change in the vicinity of the fluorescent chromophore.

A new crystal form of human histidine triad nucleotide-binding protein 1 (hHINT1) in complex with adenosine 5′-monophosphate at 1.38 Å resolution

The atomic structure of a new crystal form of the human histidine triad nucleotide-binding protein 1 (hHINT1)–AMP complex is reported at 1.38 Å resolution.

Binding of the unreactive substrate analog L-2-amino-3-guanidinopropionic acid (dinor-L-arginine) to human arginase I

Crystal structures of Mn₂²⁺- and Co₂²⁺-substituted human arginase I complexed with an unreactive analog of substrate L-arginine are described at 1.82 and 1.90 Å resolution, respectively.

Crystallization and preliminary crystallographic studies of the YafN–YafO complex from Escherichia coli

The ribosome-dependent mRNA interferase YafO from Escherichia coli belongs to a type II toxin–antitoxin (TA) system and its cognate antitoxin YafN neutralizes cell toxicity by forming a stable YafN–YafO complex. Here, the YafN–YafO complex has been expressed and crystallized.

Expression, crystallization and preliminary crystallographic data analysis of PigF, an O-methyltransferase from the prodigiosin-synthetic pathway in Serratia

The last step in the biosynthetic pathway of 4-methoxy-2,2′-bipyrrole-5-­carbaldehyde (MBC) is catalyzed by PigF, which transfers a methyl group to 4-­hydroxy-2,2′-bipyrrole-5-carbaldehyde (HBC) to form the terminal product MBC, but its catalytic mechanism is not known. To elucidate its mechanism, recombinant PigF was purified and crystallized.

Crystallization and preliminary X-ray studies of ferric uptake regulator from Magnetospirillum gryphiswaldense

Crystals of apo and SeMet ferric uptake regulator from the magnetotactic bacterium M. gryphiswaldense MSR-1 were obtained and optimized to obtain high-quality diffraction data. Diffraction data sets were collected and processed at 1.58 and 1.9 Å resolution, respectively.

Crystallization studies of the murine c-di-GMP sensor protein STING

Crystals of native and selenomethionine-labelled STING^138–344 protein from M. musculus diffracted to resolutions of 2.39 and 2.2 Å, respectively

Crystallization and preliminary X-ray diffraction analysis of a novel type of phosphoserine phosphatase from Hydrogenobacter thermophilus TK-6

A novel type of phosphoserine phosphatase from H. thermophilus TK-6 was heterologously expressed in E. coli, purified and crystallized by the sitting-drop vapour-diffusion method. The crystals obtained belonged to space group P2₁2₁2₁ and diffracted X-rays to 1.5 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of a novel GH120 β-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485

Xylosidase C from Thermoanaerobacterium saccharolyticum, the first enzyme classified as GH120 family, has been successfully crystallized. The crystal diffracted to a resolution of 2.2 Å.

Crystallization and preliminary X-ray analysis of the receiver domain of a putative response regulator, BPSL0128, from Burkholderia pseudomallei

The receiver domain of a putative response regulator from B. pseudomallei, BPSL0128, has been crystallized in a form suitable for X-ray analysis.

Cloning, expression, purification and preliminary X-ray analysis of a putative metagenome-derived lipase

A putative lipase, LipS, from the soil metagenome of an environmental sample taken at the Biocenter Klein Flottbek, Hamburg, Germany was cloned, expressed and purified. Two different constructs of the protein were crystallized in a search for well diffracting crystals. Preliminary X-ray analyses indicated that crystals in space groups P4₂2₁2 and P4 diffracted X-ray radiation to 2.8 and 2.0 Å resolution, respectively.

Crystallization and preliminary crystallographic analysis of the C-terminal domain of MamM, a magnetosome-associated protein from Magnetospirillum gryphiswaldense MSR-1

The C-terminal domain of the cation diffusion facilitator MamM has been successfully constructed, purified and crystallized. Diffraction data was collected to to a resolution of 2.0 Å.

Purification, crystallization and preliminary crystallographic analysis of banyan peroxidase

Crystals of a new peroxidase, named banyan peroxidase, from the latex of F. benghalensis belonged to the trigonal space group P3₂21 and provided X-ray diffraction data to a resolution of 1.66 Å. The anomalous signal of the intrinsic iron and calcium ions was sufficent for phasing by SAD.

Crystallization and preliminary X-ray diffraction analysis of three myotoxic phospholipases A₂ from Bothrops brazili venom

Two myotoxic and noncatalytic Lys49-phospholipases A₂ (braziliantoxin-II and MT-II) and a myotoxic and catalytic phospholipase A₂ (braziliantoxin-III) from B. brazili were crystallized. X-ray diffraction data sets were collected and molecular-replacement solutions were obtained.

Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline

As-p18, an unusual fatty-acid-binding protein from a parasitic nematode, was expressed in bacteria, purified and crystallized. The use of a microfocus beamline was essential for data collection.

Purification, crystallization and preliminary X-ray analysis of the IgV domain of human nectin-4

Nectin-4 belongs to a family of immunoglobulin-like cell adhesion molecules and is highly expressed in cancer cells. Recently, nectin-4 was found to be a receptor of measles virus and the IgV domain sustains strong binding to measles virus H protein. In this study, the successful expression and purification of human nectin-4 V domain (nectin-4v) is reported

Purification, crystallization and preliminary X-ray analysis of the DNA-binding domain of AdpA, the central transcription factor in the A-factor regulatory cascade in the filamentous bacterium Streptomyces griseus, in complex with a duplex DNA

The DNA-binding domain of AdpA, the central transcription factor in the A-factor regulatory cascade in the filamentous bacterium S. griseus, was heterologously expressed, purified and crystallized in complex with a duplex DNA by the sitting-drop vapour-diffusion method. The best crystal diffracted X-rays to 2.8 Å resolution and belonged to space group C222₁.

Characterization of crystals of an antibody-recognition fragment of the cancer differentiation antigen mesothelin in complex with the therapeutic antibody MORAb-009

The therapeutic antibody MORAb-009 disrupts the interaction of mesothelin and the ovarian cancer antigen CA-125. Crystals have been grown of the Fab fragment derived from MORAb-009 and of its complex with an N-terminal fragment of mesothelin.

Cloning, purification, crystallization and preliminary X-ray diffraction studies of Escherichia coli PapD-like protein (EcpD)

The Escherichia coli PapD-like protein (EcpD), from uropathogenic Escherichia coli (UPEC), which is a periplasmic chaperon of Yad fimbriae was cloned, overexpressed, purified and crystallized. The crystals obtained diffracted X-rays to 1.67 Å resolution and belonged to space group C222₁.

Expression, purification, crystallization and preliminary X-ray analysis of 4-hy­droxy-3-methyl-2-keto-pentanoate aldolase (asHPAL) from Arthrobacter simplex strain AKU 626

asHPAL, a member of the HpaI/HpcH subfamily of class II aldolases, was expressed, purified and crystallized in the absence and presence of 2-ketobutyrate as one of its substrates using the sitting-drop vapour-diffusion method. asHPAL crystals grown without and with 2-ketobutyrate diffracted to 1.60 and 1.55 Å resolution, respectively.

Crystallization and X-ray diffraction analysis of the ternary complex of the C-terminal domain of human REV1 in complex with REV7 bound to a REV3 fragment involved in translesion DNA synthesis

Crystallization of human REV1–REV7–REV3 ternary complex involved in translesion DNA synthesis.

Expression, purification and preliminary crystallographic analysis of Drosophila melanogaster lysosomal α-mannosidase

The purification and crystallization of the D. melanogaster lysosomal α-mannosidase is reported. The plate-like crystals belonged to the orthorhombic space group P2₁2₁2₁ and diffracted to 3.5 Å resolution.

Crystallization and preliminary X-ray analysis of Pac17 from the pacidamycin-biosynthetic cluster of Streptomyces coeruleorubidus

Crystals of Pac17, an enzyme from the pacidamycin-biosynthetic cluster of S. coeruleorubidus, were obtained and native X-ray data were recorded to a maximum resolution of 1.9 Å.

Crystallization and preliminary X-ray diffraction analysis of a fatty-acid metabolism regulatory protein, FadR, from Bacillus halodurans

Trigonal crystals of FadR from B. halodurans have been obtained. X-ray data were collected to 2.05 Å resolution using synchrotron radiation.

Preliminary crystallographic analysis of glyceraldehyde-3-phosphate de­hydrogenase 3 from Saccharomyces cerevisiae

The crystallization and preliminary X-ray diffraction analysis of glyceraldehyde-3-phosphate dehydrogenase 3 from S. cerevisiae is reported.

Structure determination by multiple-wavelength anomalous dispersion (MAD) at the Pr L_III edge

A successful MAD experiment has been conducted at the Pr L_III edge on HZB beamline BL14.2.

Crystallization of Pseudomonas aeruginosa AmrZ protein: development of a comprehensive method for obtaining and optimization of protein–DNA crystals

Crystallization of the complex of the transcription factor AmrZ with DNA was accomplished through the combination of established and newly developed methods. Here, a general method to obtain and optimize crystals of protein–DNA complexes consisting of these combined procedures is described.

Single-drop optimization of protein crystallization

A device has been developed to optimize crystal-growth conditions by experiments in a single drop.