Structure of the catalytic domain of the Tannerella forsythia matrix metallopeptidase karilysin in complex with a tetrapeptidic inhibitor

The high-resolution crystal structure of the complex between the catalytic domain of karilysin and the tetrapeptide SWFP has been determined.

Structure of the Yersinia pestis tip protein LcrV refined to 1.65 Å resolution

Here, the crystal structure of Yersinia pestis tip protein LcrV is reported at a resolution of 1.65 Å.

The structure of the CARD8 caspase-recruitment domain suggests its association with the FIIND domain and procaspases through adjacent surfaces

The structure of the CARD8 caspase-recruitment domain suggests that its regulatory functions may be mediated by electrostatic attractions between the CARD8 CARD and its partner domains.

Expression, purification, crystallization and X-ray crystallographic studies of different redox states of the active site of thioredoxin 1 from the whiteleg shrimp Litopenaeus vannamei

Analysis of the different redox states of the catalytic cysteines in four crystallographic structures of thioredoxin 1 from the Pacific whiteleg shrimp L. vannamei highlights their reactivity and corroborates the existence of a structural dimer mediated by an interface of 12 residues which includes a disulfide bridge between the Cys73 residues of each monomer.

Cocrystal structure of the ICAP1 PTB domain in complex with a KRIT1 peptide

The cocrystal structure of the PTB domain of integrin cytoplasmic domain-associated protein-1 (ICAP1) in complex with a 29-residue fragment of Krev/Rap1 interaction trapped-1 (KRIT1) has been determined.

Triosephosphate isomerase is a common crystallization contaminant of soluble His-tagged proteins produced in Escherichia coli

Crystals of E. coli triosephosphate isomerase were obtained as a contaminant and its structure was determined to 1.85 Å resolution.

Crystallization and preliminary X-ray analysis of a UDP-MurNAc-tripeptide D-alanyl-D-alanine-adding enzyme (PaMurF) from Pseudomonas aeruginosa

P. aeruginosa UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase (MurF) was crystallized in the presence of substrate and diffraction data were collected to 1.92 Å resolution.

Expression, purification, crystallization and preliminary X-ray structure analysis of wild-type and L(M196)H-mutant Rhodobacter sphaeroides reaction centres

The expression, purification, crystallization and preliminary crystallographic characterization of wild-type and L(M196)H-mutant reaction centres of Rba. sphaeroides strain RV are reported.

Purification, crystallization and preliminary X-ray analysis of the strawberry allergens Fra a 1E and Fra a 3 in the presence of catechin

The crystallization and preliminary X-ray diffraction analysis of the strawberry PR-10 proteins Fra a 1E and Fra a 3 in the presence of the natural flavonoid (+)-catechin are presented.

Purification, crystallization and preliminary crystallographic analysis of 3-hydroxyacyl-CoA de­hydrogenase from Caenorhabditis elegans

The 3-hydroxyacyl-CoA dehydrogenase from Ceanorhabditis elegans was overexpressed, purified and crystallized in two different space groups (P1 and P2₁2₁2₁). The molecular dimers found in one asymmetric unit from both space groups are identical but each packs in a distinct manner.

Preliminary X-ray crystallographic studies of an N-terminal domain of unknown function from a putative glycosyltransferase from Streptococcus parasanguinis

A streptococcal domain of unknown function 1792 has been crystallized.

Expression, purification and preliminary crystallographic analysis of the T6SS effector protein Tse3 from Pseudomonas aeruginosa

Tse3, one of the effectors of the type VI secretion system in Pseudomonas aeruginosa, has been crystallized and diffracted to 1.5 Å resolution.

Expression, crystallization and preliminary X-ray crystallographic analysis of aldehyde de­hydrogenase (ALDH) from Bacillus cereus

Aldehyde dehydrogenase (ALDH) catalyses the oxidation of aldehydes using NAD(P)⁺ as a cofactor. The aldh gene from B. cereus was cloned; the protein was expressed, purified and crystallized, and a preliminary X-ray crystallography analysis was performed.

Crystallization and preliminary X-ray diffraction analysis of the TetR-family transcriptional repressor YhgD from Bacillus halodurans

Triclinic crystals of YhgD from B. halodurans have been obtained. X-ray data have been collected to 1.9 Å resolution using synchrotron radiation.

Cloning, overexpression, purification and crystallization of the CRN12 coiled-coil domain from Leishmania donovani

The L. donovani coronin CRN12 coiled-coil domain (5.8 kDa) was cloned, overexpressed and purified to homogeneity. Crystals of recombinant L. donovani coronin CRN12 coiled-coil domain were grown by vapour diffusion using a hanging-drop setup.

Crystallization and initial X-ray data of abscisic acid receptor PYL3 in the presence of (−)-ABA

The complex of the abscisic acid receptor PYL3 with (−)-ABA was crystallized and refined to obtain high-quality diffraction data. Diffraction data were collected and processed at 2.65 Å resolution.

Purification, crystallization and preliminary X-ray crystallographic analysis of the lumenal domain of the ER-vesicle protein Erv41p from Saccharomyces cerevisiae

The lumenal domain of Erv41p from S. cerevisiae has successfully been expressed, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.0 Å.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of a core fragment of FlgG, a bacterial flagellar rod protein

A core fragment of Salmonella FlgG has been expressed, purified and crystallized, and X-ray diffraction data sets were collected to 2.0 Å resolution.

Crystallization and preliminary crystallographic analysis of an Escherichia coli-selected mutant of the nuclease domain of the metallonuclease colicin E7

An N-terminally truncated mutant of the colicin E7 nuclease domain was crystallized and diffraction data set was collected to 1.6 Å resolution.

SAD phasing towards structure determination of a thermostable Rieske ferredoxin with a novel stabilizing di­sulfide bridge

Crystallization and in-house SAD phasing unveils the structure of a unique thermophilic Rieske ferredoxin with a stabilizing disulfide bridge.

Expression, purification and crystallization of the C-terminal LRR domain of Streptococcus pyogenes protein 0843

The C-terminal LRR domain of S. pyogenes protein 0843 was overexpressed in E. coli, purified and crystallized. A complete data set to 1.59 Å resolution was collected using synchrotron radiation.

Crystallization and preliminary X-ray analysis of the CRP–cAMP–DNA (full length) complex

The Escherichia coli cyclic AMP receptor protein (CRP) crystals were obtained and diffracted at a 2.9 Å resolution, which belonged to the space group P3₁21, with unit-cell parameters a = b = 76.03, c = 144.00 Å. The asymmetric unit was found to contain one protein molecule and a half 38 bp full-length double-stranded DNA fragment with a Matthews coefficient of 2.62 ų Da⁻¹ and a solvent content of 53.14%.

Cloning, expression, purification and preliminary X-ray diffraction studies of a mycobacterial protein implicated in bacterial survival in macrophages

The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of MSMEG_5817 is described.

Cloning, expression, purification, crystallization and preliminary X-ray analysis of EaLsc, a levansucrase from Erwinia amylovora

EaLsc, a levansucrase from E. amylovora, has been cloned, expressed, purified and crystallized. X-ray crystallographic analysis and data collection to 2.77 Å resolution have been carried out. The structure was solved by molecular replacement and refinement is in progress.

Purification, crystallization and preliminary X-ray diffraction analysis of Omp6, a protoilludene synthase from Omphalotus olearius

The expression, purification and crystallization of Omp6, a protoilludene synthase from O. olearius, are described.

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of Rv2606c from Mycobacterium tuberculosis H37Rv

The pyridoxal biosynthesis lyase (PdxS) from Mycobacterium tuberculosis was crystallized and diffracted to a resolution of 1.8 Å. The crystals were found to belong to space group I222 or I2₁2₁2₁, with unit-cell parameters a = 110.75, b = 126.08, c = 180.82 Å, α = β = γ = 90°.

Purification, crystallization and preliminary X-ray analysis of the effector domain of AlsR, an LysR-type transcriptional regulator from Bacillus subtilis

The cloning, purification, crystallization and preliminary crystallographic analysis of the effector-binding domain of the transcriptional regulator AlsR from B. subtilis are described.

Crystallization and preliminary X-ray diffraction analysis of the Cmr2–Cmr3 subcomplex in the CRISPR–Cas RNA-silencing effector complex

The Cmr2–Cmr3 subcomplex from P. furiosus was co-crystallized with 3′-AMP. X-ray diffraction data for the crystals were collected to 2.6 Å resolution using a synchrotron-radiation source.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of Bifidobacterium adolescentis xylose isomerase

The xylose isomerase enzyme from B. adolescentis was expressed in E. coli, purified and crystallized. A native X-ray diffraction data set was collected to 1.7 Å resolution.