Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens

Ye, F.; Wang, C.; Fu, Q.; Zhang, L.; Gao, Y.G.

doi:10.1107/S2053230X15012881

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Expression and purification of SghA (a) and SghR (b). (a) Lane M, molecular-weight marker (labelled in kDa); lanes 1 and 2, supernatant and pellet after cell extraction, respectively; lane 3, flowthrough after Ni–NTA affinity binding; lanes 4 and 5, after elution from Ni–NTA affinity column; lane 6, purified SghA protein after gel-filtration chromatography. (b) Lane M, molecular-weight marker (labelled in kDa); lanes 1 and 2, total cells before and after IPTG induction, respectively; lane 3, flowthrough after Ni–NTA affinity binding; lanes 4 and 5, supernatant and pellet after cell extraction, respectively; lane 6, after elution from Ni–NTA purification; lane 7, purified SghR after gel-filtration chromatography.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 71| Part 9| August 2015| Pages 1139-1145

doi:10.1107/S2053230X15012881

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