Structure of the 14-3-3ζ–LKB1 fusion protein provides insight into a novel ligand-binding mode of 14-3-3

The crystal structure of a 14-3-3ζ–LKB1 fusion protein provides a novel potential binding mode of 14-3-3 to its ligands.

Crystallographic studies of the structured core domain of Knr4 from Saccharomyces cerevisiae

The potentially structured core domain of the intrinsically disordered protein Knr4, residues 80–340, was expressed and crystallized using native and selenomethionine-labelled proteins.

Structure of RizA, an L-amino-acid ligase from Bacillus subtilis

The structure of the L-amino-acid ligase RizA was determined at 2.8 Å resolution.

X-ray crystallographic studies of the middle part of the human synaptonemal complex protein 1 coiled-coil domain

The middle of syneptonemal complex protein 1, which is composed of a coiled-coil domain, was purified and crystallized. The crystals were found to belong to space group I4, with unit-cell parameters a = 41.95, b = 41.95, c = 318.78 Å. The crystals were obtained at 293 K and diffracted to a resolution of 3.0 Å.

Crystallization and crystallographic studies of kallistatin

The crystallization of human kallistatin in the relaxed conformation is reported.

Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens

The crystallization of the novel virulence factors SghA and SghR is reported.

The structure of Tim50(164–361) suggests the mechanism by which Tim50 receives mitochondrial presequences

The Tim50 crystal structure indicates that the IMS domain of Tim50 exhibits significant structural plasticity within the putative presequence-binding groove.

Cleavage of nicotinamide adenine dinucleotide by the ribosome-inactivating protein from Momordica charantia

High-resolution structural analyses of the N-glycosidase ribosome-inactivating protein from seeds of M. charantia show that the enzyme preferentially cleaves nicotinamide from the oxidized form of the coenzyme NADP.

Crystallization and preliminary X-ray crystallographic analysis of a nonstructural protein 15 mutant from Human coronavirus 229E

This article describes preliminary crystallographic data for the I26A/N52A mutant of nonstructural protein 15 from Human coronavirus 229E.

Crystals of the Arp2/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASP binding sites

The actin-polymerizing protein complex Arp2/3 was crystallized in two new space groups. The new electron-density maps from these new crystal forms reveal some details that may help to elucidate how nucleation-promoting factors bind to the complex.

Structure of the N-terminal dimerization domain of CEACAM7

The crystal structure of the dimerization domain of CEACAM7, a human cell adhesion protein, was determined at 1.47 Å resolution and the K_dimerization was measured by analytical ultracentrifugation.

Structure of the SPRY domain of the human RNA helicase DDX1, a putative interaction platform within a DEAD-box protein

The structure of the SPRY domain of the human RNA helicase DDX1 was determined at 2.0 Å resolution. The SPRY domain provides a putative protein–protein interaction platform within DDX1 that differs from other SPRY domains in its structure and conserved regions.

Expression, purification, crystallization and X-ray diffraction studies of the molecular chaperone prefoldin from Homo sapiens

The six subunits of human prefoldin were overexpressed in E. coli. Prefoldin was reconstituted, purified and crystallized. X-ray diffraction data were collected to 4.7 Å resolution.

Crystallization and X-ray diffraction analysis of a putative bacterial class I labdane-related diterpene synthase

The subcloning, expression, purification and crystallization of a putative class I labdane-related diterpene synthase identified by genome mining in the genome sequence of a Mexican actinobacterium is reported.

Crystallization and X-ray diffraction studies of a two-domain laccase from Streptomyces griseoflavus

In this study, a recombinant two-domain laccase from S. griseoflavus was successfully expressed and purified, and crystals were obtained that diffracted to a resolution of 2.0 Å.