Structure of DnmZ, a nitrososynthase in the Streptomyces peucetius anthracycline biosynthetic pathway

The anthracycline biosynthetic enzyme DnmZ has been structurally characterized in ligand-free and thymidine diphosphate-bound forms.

Structure of the response regulator RPA3017 involved in red-light signaling in Rhodopseudomonas palustris

The crystal structure of the response regulator RPA3017 from the photosynthetic bacterium R. palustris has been determined at 1.9 Å resolution. RPA3017 is part of a two-component system that involves two tandem bacteriophytochromes involved in red-light signaling.

Purification, crystallization, crystallographic analysis and phasing of the CRISPR-associated protein Csm2 from Thermotoga maritima

Csm2 is a major component of type IIIA CRISPR ribonucleoprotein complexes that confer prokaryotes with immunity against phages and plasmids via an RNA-guided interference mechanism. The Csm2 protein from T. maritima was recombinantly expressed, purified and crystallized, and its structure was solved via cadmium single-wavelength anomalous diffraction phasing.

Analysis of crystallization data in the Protein Data Bank

In a large-scale study using data from the Protein Data Bank, some of the many reported findings regarding the crystallization of proteins were investigated.

Structure and binding properties of a cameloid nanobody raised against KDM5B

KDM5B is an important new putative cancer-therapy target. Here, the binding properties and crystal structure of a nanobody raised against KDM5B are described.

Crystallization and initial crystallographic analysis of covalent DNA-cleavage complexes of Staphyloccocus aureus DNA gyrase with QPT-1, moxifloxacin and etoposide

The antibacterial compound QPT-1, the antibacterial drug moxifloxacin and the anticancer drug etoposide were each co-crystallized with DNA and S. aureus DNA gyrase. The base pairs at the ends of the DNA duplexes were modified to optimize the crystal contacts and to give diffraction data to between 2.45 and 3.15 Å resolution from six DNA-complex crystals.

Purification, crystallization and X-ray crystallographic analysis of human RAB11(S20V), a constitutively active GTP-binding form

RAB11(S20V), a constitutively active GTP-binding form of RAB11, was purified and crystallized. Crystals were obtained at 293 K and diffracted to a resolution of 2.4 Å; they belonged to space group I4, with unit-cell parameters a = 74.11, b = 74.11, c = 149.44 Å.

Structure of the HECT domain of human WWP2

The crystal structure of the HECT domain of WWP2, an E3 ubiquitin–protein ligase, has been determined at 2.5 Å resolution and revealed a compact inverted T-shaped conformation.

Extension of resolution and oligomerization-state studies of 2,4′-di­hydroxyaceto­phenone dioxygenase from Alcaligenes sp. 4HAP

The resolution of the structure of 2,4′-dihydroxyacetophenone dioxygenase has been extended to 1.88 Å and it has been demonstrated by gel filtration and analytical ultracentrifugation that in situ chymotrypsinolysis, which is required for crystallization, converts the enzyme from a tetrameric form to a dimeric form. A putative model of the tetramer obtained by symmetric docking studies closely resembles the tetrameric assembly of the homologous RmlC-like cupin protein from R. eutropha.

Structures of exoglucanase from Clostridium cellulovorans: cello­tetraose binding and cleavage

Cellotetraose binding and cleavage by GH48 ExgS (glycosyl hydrolase 48 cellobiohydrolase S).

The structure of the death receptor 4–TNF-related apoptosis-inducing ligand (DR4–TRAIL) complex

The structure of the death receptor 4–TNF-related apoptosis-inducing ligand (DR4–TRAIL) complex is presented and compared with those of DR5–TRAIL complexes. Isothermal titration calorimetry data are presented that demonstrate differences in the interactions of DR4 and DR5 with TRAIL.

Crystallization and preliminary X-ray diffraction studies of frutalin, an α-D-galactose-specific lectin from Artocarpus incisa seeds

Frutalin, an α-D-galactose-binding lectin of biomedical interest from A. incisa seeds, has been crystallized and its preliminary X-ray diffraction analysis at 1.81 Å resolution is reported.

Crystallization and X-ray analysis of the transcription-activator protein C1 of bacteriophage P22 in complex with the P_RE promoter element

Crystallization and preliminary data collection to 2.2 Å resolution is reported for the C1 protein of Salmonella phage P22 in complex with a 23-mer DNA.

An active site–tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase

Citrate synthase from the thermophilic euryarchaeon T. acidophilum fused to a hexahistidine tag was purified and biochemically characterized. The structure of the unliganded enzyme at 2.2 Å resolution contains tail–active site contacts in half of the active sites.

Structural analysis of SepL, an enteropathogenic Escherichia coli type III secretion-system gatekeeper protein

The X-ray crystal structure of the enteropathogenic E. coli gatekeeper protein SepL contains three X-bundle domains.

Structure of a catalytic dimer of the α- and β-subunits of the F-ATPase from Paracoccus denitrificans at 2.3 Å resolution

The structure of the αβ heterodimer of the F-ATPase from the α-proteobacterium P. denitrificans has been determined at 2.3 Å resolution. It corresponds to the `open' or `empty' catalytic interface found in other F-ATPases.

1.25 Å resolution structure of an RNA 20-mer that binds to the TREX2 complex

The 1.25 Å resolution crystal structure of a 20 nt ribonucleotide that binds to the TREX2 complex with high affinity shows a double-stranded RNA duplex that contains C–A, U–U and C–C noncanonical base pairings together with canonical Watson–Crick A–U and G–C pairs and a G–U wobble.

Overexpression, purification and crystallization of the response regulator NsrR involved in nisin resistance

The response regulator NsrR from S. agalactiae, which is involved in nisin resistance, was crystallized, yielding crystals that diffracted to 1.4 Å resolution.

High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thi­amine pyrophosphate

The crystal structure of L. salivarius transketolase has been determined to high resolution in the presence and absence of thiamine pyrophosphate. The structures are presented with a brief comparison with other known transketolase structures.

Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine

The crystal structure of the complex of porcine carboxypeptidase B with an arginine transition-state analogue is reported for the first time.

Structure of the dual-function fructose-1,6/sedoheptulose-1,7-bis­phosphatase from Thermosynechococcus elongatus bound with sedoheptulose-7-phosphate

The X-ray crystal structure of the dual-function fructose-1,6/sedoheptulose-1,7-bisphosphatase has been solved to 2.3 Å resolution in the presence of sedoheptulose-7-phosphate and the absence of adenosine monophosphate.

Stabilization of porcine pancreatic elastase crystals by glutaraldehyde cross-linking

Crystals of porcine pancreatic elastase were cross-linked with glutaraldehyde, soaked with a novel inhibitor and characterized. This method enabled the soaking of a hydrophobic ligand into the protein crystals.

A sucrose-binding site provides a lead towards an isoform-specific inhibitor of the cancer-associated enzyme carbonic anhydrase IX

An engineered human carbonic anhydrase IX (CA IX) mimic was co-crystallized with the disaccharide sucrose. Its binding provides a novel `interaction' site that can be used to design targeted inhibitors of CA IX, a key marker of hypoxic tumors.

Formulation screening by differential scanning fluorimetry: how often does it work?

Over 250 proteins have been subjected to a standard dye-based thermal melt analysis, and the results suggest that at least a third of soluble protein samples can be made significantly more stable by changing the composition of their formulation.