Crystal structure of truncated human coatomer protein complex subunit ζ1 (Copζ1)

Selective targeting of the ζ1 subunit of the human coatomer protein complex I (Copζ1) is a promising avenue for anticancer therapy, as knockouts of ζ1 have been shown to specifically kill both proliferating and nondividing tumour-cell populations. In order to provide a structural basis for rational drug design, the high-resolution crystal structure of Copζ1 is reported.

Crystal structure of the ATPase-C domain of the chromatin remodeller Fun30 from Saccharomyces cerevisiae

The C-terminal domain (residues 780–1122) of the ATPase motor Fun30 from S. cerevisiae has been crystallized and the structure was determined at a resolution of 1.95 Å. A helix-bundle insertion in the ATPase domain was identified to be specific to the Fun30 subfamily.

Crystal structure of an IclR homologue from Microbacterium sp. strain HM58-2

The crystal structure of an IclR homologue from Microbacterium sp. strain HM58-2 was determined at 2.1 Å resolution. It revealed a unique tetramer conformation, which may represent one of the multiple conformations in transcriptional regulation.

X-ray crystal structure of a malonate-semi­aldehyde de­hydrogenase from Pseudomonas sp. strain AAC

The crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC has been determined to a nominal resolution of 2.95 Å.

Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC

The crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC has been determined to a resolution of 2.07 Å.

Structure of a fungal form of aspartate-semi­aldehyde de­hydrogenase from Aspergillus fumigatus

The structure of a new fungal form of aspartate-semialdehyde dehydrogenase from A. fumigatus reveals differences in secondary-structural features that will be exploited for species-selective antibiotic development.

Recombinant hepatocyte growth factor activator inhibitor 1: expression in Drosophila S2 cells, purification and crystallization

The extracellular domain of human hepatocyte growth factor activator inhibitor 1 was expressed in Drosophila S2 cells. The recombinant protein was crystallized using ammonium sulfate as precipitant and the crystals diffracted to 3.8 Å resolution, with unit-cell parameters a = b = 95.42, c = 124.50 Å.

Expression, purification and crystallization of a protein resulting from the inversion of the amino-acid sequence of a helical bundle

To explore the sequence–structure relationships of four-α-helical bundles, the revRM6 protein, which has been engineered with the exact inverse amino-acid sequence of a hyperthermophilic helical bundle, was expressed, purified and crystallized and X-ray diffraction data were collected.

Structural characterization of the Streptococcus pneumoniae carbohydrate substrate-binding protein SP0092

The crystal structure of SP0092 was determined at 1.61 Å resolution and reveals a domain-swapped dimer with the monomer subunit in a closed conformation in the absence of ligand.