Crystal structure of the DNA sequence d(CGTGAATTCACG)₂ with DAPI

The interaction of DAPI with d(CGTGAATTCACG) results in displacement of the ordered spine waters and confers hydrophobic behaviour on the DNA.

HicAB toxin–antitoxin complex from Escherichia coli: expression and crystallization

Two forms of the HicAB toxin–antitoxin complex from E. coli were purified and crystals were obtained of one of the forms.

3,6-Anhydro-L-galactonate cycloisomerase from Vibrio sp. strain EJY3: crystallization and X-ray crystallographic analysis

3,6-Anhydro-L-galactonate cycloisomerase (ACI) is a second-step enzyme in the metabolic pathway of 3,6-anhydro-L-galactose. Structural analysis of ACI is crucial in order to elucidate its function, substrate specificity and reaction mechanism at the molecular level.

Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana

The crystal structure of zearalenone hydrolase from C. batiana is reported.

The LRR-Roc-COR module of the Chlorobium tepidum Roco protein: crystallization and X-ray crystallographic analysis

The Roco protein from the bacterium C. tepidum has been used as a model system to investigate the structure and mechanism of the Roco protein family. Here, the crystallization and crystallographic analysis of the LRR-Roc-COR construct of the C. tepidum Roco protein are reported.

Protein crystallization and initial neutron diffraction studies of the photosystem II subunit PsbO

Crystals of PsbO were grown in capillaries using an agarose-plug setup and were cross-linked using glutaraldehyde. Initial neutron diffraction data were collected to 2.3 Å resolution on the MaNDi instrument at the Spallation Neutron Source.

Ectodomain of plasmodesmata-localized protein 5 in Arabidopsis: expression, purification, crystallization and crystallographic analysis

Plasmodesmata-localized protein 5 (PDLP5) is a member of the cysteine-rich receptor-like plasmodesmata-localized proteins and is involved in the regulation of plasmodesmata permeability. The ectodomain of PDLP5, which possesses two DUF26 domains, was expressed, purified and crystallized by the hanging-drop vapour-diffusion method.

Production, crystallization and structure determination of a mycobacterial glu­cosylglycerate hydrolase

Mycobacterial glucosylglycerate hydrolase is a highly conserved enzyme that is involved in recovery from nitrogen starvation. Here, the production, crystallization and structure solution of Mycobacterium hassiacum glucosylglycerate hydrolase using two-wavelength anomalous diffraction of selenomethionine-substituted crystals are presented.