Structural analysis of the chicken FANCM–MHF complex and its stability

Three crystals of MHF and its complex with FANCM were obtained, and structural analysis revealed not only their structures but the unexpected release of FANCM from MHF. The biochemical stability of the FANCM–MHF complex was analyzed and it was found that an oxidative environment and organic solvent promote the aggregation and dissociation of the complex.

Expression, purification and crystallization of the N-terminal Solanaceae domain of the Sw-5b NLR immune receptor

Protein crystallization and X-ray data collection of the N-terminal Solanaceae domain of the Sw-5b NLR immune receptor are described.

Crystal structure of human V-1 in the apo form

The crystal structure of human V-1 in the apo form was determined at 2.3 Å resolution. The results demonstrate that V-1 is a constitutively active inhibitor of actin-capping protein.

Using yeast surface display to engineer a soluble and crystallizable construct of hematopoietic progenitor kinase 1 (HPK1)

Yeast surface display was applied to a library of hematopoietic progenitor kinase 1 (HPK1) kinase domain variants in order to select those with an improved expression level and solubility. The HPK1 variant with the most improved properties contained two mutations and crystallized readily in complex with several small-molecule inhibitors.

A fully automated crystallization apparatus for small protein qu­antities

An improved, fully automated protein crystallization and monitoring system, PXS2, can perform crystallization using smaller protein samples than the previous version. In addition, PXS2 can be used for membrane-protein crystallization using the bicelle and LCP methods.