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Figure 5
Crystals of membrane-protein samples. (a) Crystals obtained by the bicelle method. RxR (left) and PorB (right) crystals were grown under conditions consisting of 0.05 M HEPES pH 7.5, 2.5 M ammonium sulfate and of 35%(v/v) MPD, 0.1 M Tris pH 8.5, 0.2 M ammonium sulfate, respectively. The premixed bicelle solutions were purchased from Molecular Dimensions (Sheffield, UK). (b) Crystals obtained by the LCP method. RxR (left) and PorB (right) crystals were grown under conditions consisting of 0.1 M HEPES pH 7.5, 1 M sodium sulfate, 0.05 M cadmium sulfate and of 20%(v/v) PEG 300, 10%(w/v) glycerol, 0.1 M imidazole, 1 M ammonium sulfate, respectively. |